Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Hes1 antibody
See all Hes1 products (8) ...
Description
Rabbit polyclonal to Hes1
Specificity
This antibody reacts specifically with mouse 30 kDa Hes1 protein.
Tested applications
IHC-Fr, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide derived from the C-terminal domain of (Mouse) Hes1 protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration.
IHC-P: Use at an assay dependent dilution.
WB: 1/200 - 1/2000.Predicted molecular weight: 30 kDa.
Target
Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ14469
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
Database links
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: Q14469 Human
- SwissProt: P35428 Mouse
- SwissProt: Q04666 Rat
- Unigene: 250666 Human
see all
Anti-Hes1 antibody images:
Immunohistochemistry (Frozen sections) - Hes1 antibody (ab71559)
ab71559 staining Hes1 in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone/methanol (1:1), permeabilized with PBS + triton X100 (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Western blot - Hes1 antibody (ab71559)
All lanes : Anti-Hes1 antibody (ab71559)
Lane 1 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 2 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 3 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 4 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 5 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 6 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 7 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Lane 8 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Predicted band size : 30 kDa
Actin blot given as control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hes1 antibody (ab71559)
ab71559 staining Hes1 in small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.
This image is courtesy of an Anonymous Abreview.
References for Anti-Hes1 antibody (ab71559)
This product has been referenced in:
- Karbowniczek Met al. The evolutionarily conserved TSC/Rheb pathway activates Notch in tuberous sclerosis complex and Drosophila external sensory organ development. J Clin Invest 120:93-102 (2010). WB.Read more (PubMed: 20038815) »
- Medic S & Ziman M PAX3 expression in normal skin melanocytes and melanocytic lesions (naevi and melanomas). PLoS One 5:e9977 (2010). IHC-P; Human.Read more (PubMed: 20421967) »
See all 3 publications for this product
Publishing research using ab71559? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"