Recombinant Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673]
See all Heterogeneous Nuclear Ribonucleoprotein (A1-like) primary antibodies -
Description
Rabbit monoclonal [EPR13673] to Heterogeneous Nuclear Ribonucleoprotein (A1-like) -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, K562, A431 and 293 cell lysates; Human cervix squamous cell carcinoma tissue; K562 and 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13673 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/5000 - 1/10000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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Notes |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/5000 - 1/10000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/250. |
Target
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Relevance
The function remains unknown. -
Database links
- Entrez Gene: 144983 Human
- SwissProt: Q5TBS2 Human
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Alternative names
- HNRPA1L antibody
- LOC144983 antibody
- MGC102957 antibody
- RP11-78J21.1 antibody
Images
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All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody [EPR13673] (ab180124) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa -
Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(HRP) secondary and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent analysis of 293 (paraformaldehyde-fixed cells, 4%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like) with ab180124 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/250 dilution and counterstained with DAPI (blue).
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Intracellular flow cytometric analysis of K562 cells (paraformaldehyde-fixed, 2%) labeling Heterogeneous Nuclear Ribonucleoprotein (A1-like)with ab180124 at 1/10 dilution or a rabbit IgG (negative), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab180124 has been referenced in 1 publication.
- Lim J et al. The histone variant H3.3 G34W substitution in giant cell tumor of the bone link chromatin and RNA processing. Sci Rep 7:13459 (2017). PubMed: 29044188