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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab34222? Please let us know so that we can cite the reference in this datasheet
ab34222 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Anti-Hex antibody - ChIP Grade (ab34222)
Anti-Hex antibody - ChIP Grade (ab34222) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Immunohistochemistry (PFA perfusion fixed frozen sections) - Hex antibody (ab34222)
ab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet
Hex antibody for IHC-FoFr in Mouse (34222)
Hex antibody for ChIP in Mouse (34222)
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