![Western blot - Hex antibody [EPR3361] (ab79392) image](#Hex-Primary-antibodies-ab79392-1.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Overview
Product name
Anti-Hex antibody [EPR3361]
See all Hex products (7) ...
Description
Rabbit monoclonal [EPR3361] to Hex
Tested applications
WB, IP, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues in human Hex
Positive control
MCF7 and A549 cell lysates.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR3361
Isotype
IgG
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Western blot - Hex antibody [EPR3361] (ab79392)
![Western blot - Hex antibody [EPR3361] (ab79392) image](/ps/datasheet/images/79/ab79392/Hex-Primary-antibodies-ab79392-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/2000.Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP
IP: 1/40IP: 1/40
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Flow Cyt
Flow Cyt: 1/20Flow Cyt: 1/20
Application notes
Is unsuitable for or IHC-P.
Target
Function
Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
Tissue specificity
Liver and promyelocytic leukemia cell line HL60.
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Developmental stage
Expressed during hematopoiesis.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ03014
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
Database links
- Entrez Gene: 3087 Human
- Omim: 604420 Human
- SwissProt: Q03014 Human
- Unigene: 118651 Human
Anti-Hex antibody [EPR3361] images:
Western blot - Hex antibody [EPR3361] (ab79392)
All lanes : Anti-Hex antibody [EPR3361] (ab79392) at 1/2000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 30 kDa
Observed band size : 30 kDa
References for Anti-Hex antibody [EPR3361] (ab79392)
ab79392 has not yet been referenced specifically in any publications.
Publishing research using ab79392? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"