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Western blot - Hex antibody (ab34222)
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Western blot - Hex antibody (ab34222)
(enlarge)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Hex antibody (ab34222)
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Product Name
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Hex antibody - ChIP Grade
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See all Hex antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Hex - ChIP Grade
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Hex. (Peptide available as ab28465.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, Pig
(Due to sequence homology)
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Tested applications
(see key)
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ChIP, IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution.
IHC-FoFr: 1/100 (See abreview).
WB: 1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
HEK293 Human embryonic kidney cell line
SHSY-5Y (Human neuroblastoma cell line)
HepG2 (Human hepatocellular liver carcinoma cell line)
NIH 3T3 (Mouse embryonic fibroblast cell line)
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Lineage specification >> Mesoderm
Developmental Biology >> Embryogenesis >> Axis formation and symmetry
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Stem Cells >> Lineage Markers >> Mesoderm
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
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Relevance
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Hex is a homeobox transcription factor expressed during hematopoiesis. Hex functions at multiple stages of development within differentiating embryoid bodies and has a role as a negative regulator of the hemangioblast and the endothelial lineage.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Hex antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Hex antibody - ChIP Grade images, references, products related to ab34222 and other tools.
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Hex antibody - ChIP Grade images:
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 Western blot - Hex antibody (ab34222)
All lanes : Hex antibody - ChIP Grade (ab34222) at 1/250 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
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Western blot - Hex antibody (ab34222)
All lanes : Hex antibody - ChIP Grade (ab34222) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Hex antibody (ab34222)
ab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary. This image is courtesy of an Abreview submitted by Dr Sophie Pezet
See Abreview
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Search PubMed (MEDLINE) for references to Hex
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Hex antibody - ChIP Grade - more information
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Customer reviews (feedback) regarding Hex antibody - ChIP Grade |
Customer FAQs regarding Hex antibody - ChIP Grade |
Protocols for Hex antibody - ChIP Grade |
Price and availability of products related to Hex antibody - ChIP Grade |
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Hex antibody - ChIP Grade - related products:
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Hex antibody - ChIP Grade - other tools:
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Contact Abcam with a Technical Enquiry about Hex antibody - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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