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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Overview
Product name
Anti-Hex antibody
See all Hex products (7) ...
Description
Rabbit polyclonal to Hex
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Rat, Cow, Dog, Pig
Immunogen
Synthetic peptide: RLKQENPQSN KKEELESLDS SCDQRQDLPS EQNKGASLDS SQCSPSPASQ , corresponding to amino acids 193-243 of Human Hex
RLKQENPQSN KKEELESLDS SCDQRQDLPS EQNKGASLDS SQCSPSPASQ
Positive control
K562 cell lysate, Human muscle tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/312500ELISA: 1/312500
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
Tissue specificity
Liver and promyelocytic leukemia cell line HL60.
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Developmental stage
Expressed during hematopoiesis.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ03014
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
Database links
- Entrez Gene: 3087 Human
- Entrez Gene: 397232 Pig
- Entrez Gene: 79237 Rat
- Omim: 604420 Human
- SwissProt: Q03014 Human
- SwissProt: Q9WV22 Rat
- Unigene: 118651 Human
- Unigene: 12188 Rat
Anti-Hex antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Hex antibody (ab42441)
Ab42441 at 4.0 µg/ml, staining Hex in human skeletal muscle cells by immunohistochemistry, parafin embedded human muscle tissue.
Western blot - Hex antibody (ab42441)
Anti-Hex antibody (ab42441) at 0.5 µg/ml + K562 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 30 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-Hex antibody (ab42441)
This product has been referenced in:
- Sripichai Oet al. Cytokine-mediated increases in fetal hemoglobin are associated with globin gene histone modification and transcription factor reprogramming. Blood 114:2299-306 (2009). WB; Human.Read more (PubMed: 19597182) »
See 1 publication for this product
Publishing research using ab42441? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"