General notesab94049 is a 293T cell transfected lysate in which Human Hexokinase 1 has been transiently over-expressed using a pCMV-Hexokinase 1 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more detailed how the transfected lysate was prepared view preparation notes
BackgroundDisease: Hexokinase deficiency
Hereditary motor and sensory neuropathy, Russe type
Domain: The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Pathway: Carbohydrate metabolism; hexose metabolism.
Similarity: Belongs to the hexokinase family.
Contains 2 hexokinase domains.
Tissue specificity: Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.