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Hexokinase 1 293T Transfected Lysate - (positive control) (ab94049)

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Overview

  • Product nameHexokinase 1 293T Transfected Lysate - (positive control)
  • General notesab94049 is a 293T cell transfected lysate in which Human Hexokinase 1 has been transiently over-expressed using a pCMV-Hexokinase 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundDisease: Hexokinase deficiency (HK deficiency) [MIM:235700]: Rare autosomal recessive disease with nonspherocytic hemolytic anemia as the predominant clinical feature. Note=The disease is caused by mutations affecting the gene represented in this entry. Domain: The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. Pathway: Carbohydrate metabolism; hexose metabolism. Similarity: Belongs to the hexokinase family. Tissue specificity: Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.

    • Applications

    Our Abpromise guarantee covers the use of ab94049 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Hexokinase 1 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Hexokinase 1 293T Transfected Lysate - (positive control) (ab94049)
      SDS-PAGE - Hexokinase 1 293T Transfected Lysate - (positive control) (ab94049)
      ab94049 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Hexokinase 1 293T Transfected Lysate - (positive control) (ab94049)
      Western blot - Hexokinase 1 293T Transfected Lysate - (positive control) (ab94049)
      All lanes : Anti-Hexokinase 1 antibody (ab55144) at 1/500 dilution

      Lane 1 : Hexokinase 1 293T Transfected Lysate - (positive control) (ab94049)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Hexokinase 1 293T Transfected Lysate - (positive control) (ab94049)

    ab94049 has not yet been referenced specifically in any publications.

    Publishing research using ab94049 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"