Anti-Hexokinase Type III antibody - C-terminal (ab71726)
Overview
- Product nameAnti-Hexokinase Type III antibody - C-terminalSee all Hexokinase Type III primary antibodies ...
- DescriptionRabbit polyclonal to Hexokinase Type III - C-terminal
- Tested applicationsELISA, IHC-P, WB more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide from the C-terminal region of human Hexokinase Type III conjugated to KLH
- Positive controlHL-60 cell lysate and human hepatocarcinoma tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityProtein G purified
- Purification notesab71726 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab71726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/1000. |
| IHC-P | IHC-P: 1/50 - 1/100. |
| WB | WB: 1/100 - 1/500. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa). |
Target
- PathwayCarbohydrate metabolism; hexose metabolism.
- Sequence similaritiesBelongs to the hexokinase family.
- DomainThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
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Database links
- Entrez Gene: 3101 Human
- Omim: 142570 Human
- SwissProt: P52790 Human
- Unigene: 411695 Human
Target information above from: UniProt accession
P52790
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ATP:D hexose 6 phosphotransferase antibodyHexokinase type III antibodyHexokinase-3 antibody
- Hexokinase3 antibodyHK III antibodyHK3 antibodyHKIII antibodyHXK3 antibodyHXK3_HUMAN antibody
see all
Anti-Hexokinase Type III antibody - C-terminal images
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Anti-Hexokinase Type III antibody - C-terminal (ab71726) at 1/100 dilution + HL-60 cell lysate at 12.5 µg
Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hexokinase Type III antibody - C-terminal (ab71726)ab71726 at 1/50 dilution, staining Hexokinase Type III in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
References for Anti-Hexokinase Type III antibody - C-terminal (ab71726)
ab71726 has not yet been referenced specifically in any publications.
