The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Carbohydrate metabolism; hexose metabolism.
Belongs to the hexokinase family.
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
Western blot - Hexokinase Type III antibody - C-terminal (ab71726)
Anti-Hexokinase Type III antibody - C-terminal (ab71726) at 1/100 dilution + HL-60 cell lysate at 12.5 µg
Predicted band size: 99 kDa Observed band size: 99 kDa Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hexokinase Type III antibody - C-terminal (ab71726)
ab71726 at 1/50 dilution, staining Hexokinase Type III in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.