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Western blot - Hey L antibody (ab61277)
(enlarge)
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Product Name
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Hey L antibody
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See all Hey L antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Hey L
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Immunogen
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Recombinant fragment with tag:
PLRRATGIIL PARRNVLPSR GASSTRRARP LERPATPVPV APSSRAARSS HIAPLLQSSS PTPPGPTGSA AYVAVPTPNS SSPGPAGRPA GAMLYHSWVS EITEIGA, corresponding to amino acids 221-327 of Human Hey L
BLAST 'PLRRATGIIL PARRNVLPSR GASSTRRARP LERPATPVPV APSSRAARSS HIAPLLQSSS PTPPGPTGSA AYVAVPTPNS SSPGPAGRPA GAMLYHSWVS ' with  or 
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Reacts with
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Hu
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Predicted to react
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Chmp, RMk
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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HEYL belongs to hairy-related bHLH transcription factor family.
This 328 amino acid protein contains the basic helix-loop-helix and orange domains conserved in HEY proteins, but it lacks the YRPW motif found in HEY1 and HEY2. HEYL shares 78% amino acid identity with the mouse protein, with most divergence in the C terminal domain. Northern blot analysis of mouse tissues detected a 3.3 kb Heyl transcript in most tissues, with a second, larger Heyl transcript also present in ovary.
HEY genes are candidates for several human or mouse disease loci.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Hey L antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Hey L antibody images, references, products related to ab61277 and other tools.
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Hey L antibody images:
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 Western blot - Hey L antibody (ab61277)
Predicted band size : 35 kDa
Western blot against tagged recombinant protein immunogen using ab61277 Hey L antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa.
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Search PubMed (MEDLINE) for references to Hey L
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Hey L antibody - more information
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Customer reviews (feedback) regarding Hey L antibody |
Customer FAQs regarding Hey L antibody |
Protocols for Hey L antibody |
Price and availability of products related to Hey L antibody |
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Hey L antibody - related products:
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Hey L antibody - other tools:
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Contact Abcam with a Technical Enquiry about Hey L antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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