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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-Hey L antibody
See all Hey L products (4) ...
Description
Mouse monoclonal to Hey L
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee, Rhesus monkey
Immunogen
Recombinant fragment with tag: PLRRATGIIL PARRNVLPSR GASSTRRARP LERPATPVPV APSSRAARSS HIAPLLQSSS PTPPGPTGSA AYVAVPTPNS SSPGPAGRPA GAMLYHSWVS EITEIGA, corresponding to amino acids 221-327 of Human Hey L
PLRRATGIIL PARRNVLPSR GASSTRRARP LERPATPVPV APSSRAARSS HIAPLLQSSS PTPPGPTGSA AYVAVPTPNS SSPGPAGRPA GAMLYHSWVS EITEIGA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - Hey L antibody (ab61277)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
HEYL belongs to hairy-related bHLH transcription factor family. This 328 amino acid protein contains the basic helix-loop-helix and orange domains conserved in HEY proteins, but it lacks the YRPW motif found in HEY1 and HEY2. HEYL shares 78% amino acid identity with the mouse protein, with most divergence in the C terminal domain. Northern blot analysis of mouse tissues detected a 3.3 kb Heyl transcript in most tissues, with a second, larger Heyl transcript also present in ovary. HEY genes are candidates for several human or mouse disease loci.
Cellular localization
Nuclear
Alternative names
- Hairy related transcription factor 3 antibody
- Hairy enhancer of split related with YRPW motif like antibody
- Hairy/enhancer of split related with YRPW motif like antibody
see all
Database links
- Entrez Gene: 26508 Human
- NCBI: NP_055386.1 Human
- Omim: 609034 Human
- SwissProt: Q5TG99 Human
- SwissProt: Q9NQ87 Human
- Unigene: 472566 Human
Anti-Hey L antibody images:
Western blot - Hey L antibody (ab61277)
Predicted band size : 35 kDa
Western blot against tagged recombinant protein immunogen using ab61277 Hey L antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa.
References for Anti-Hey L antibody (ab61277)
ab61277 has not yet been referenced specifically in any publications.
Publishing research using ab61277? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"