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ab129733 is an In-Cell ELISA (ICE) assay kit that uses quantitative immunocytochemistry to measure HIF1 alpha and BNIP3 protein levels in cultured cells. Cells are fixed in a microplate and targets of interest are detected with highly specific, well-characterized antibodies. Relative protein levels are quantified using IRDye®-labeled Secondary Antibodies and IR imaging using a LI-COR® Odyssey® or Aerius® system.
Hypoxia and the cellular response to hypoxic environment is a central topic in studies of metabolism, cancer progression and development and stem cells. A key player is the transcription factor HIF1 alpha (hypoxia inducible factor 1 alpha) which is stabilized at the protein level in response to decreased oxygen tension. HIF1 alpha then promotes transcription of a number of factors that alters cellular physiology. This Hypoxia ICE assay kit provides duplexed measurements of the transcription factor HIF1 alpha and the HIF1A responsive protein BNIP3.
Plates are available in our ICE (In-Cell ELISA) Support Pack (ab111542) which can be bought seperately.
|Components||1 x 96 tests|
|1000X IRDye-labeled Secondary Antibodies||1 x 24µl|
|100X BNIP3 Primary Antibody (Mouse)||1 x 120µl|
|100X HIF1alpha Primary Antibody (Rabbit)||1 x 120µl|
|100X Triton X-100||1 x 0.5ml|
|10X Blocking Buffer||1 x 10ml|
|10X Phosphate Buffered Saline||1 x 100ml|
|400X Tween-20||1 x 2ml|
|Janus Green Stain||1 x 17ml|
Our Abpromise guarantee covers the use of ab129733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||Use at an assay dependent concentration.|
ab129733 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"