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IL4 High Sensitivity Human ELISA Kit (ab46063)

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Overview

  • Product nameIL4 High Sensitivity Human ELISA KitSee all IL4 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 7.43pg/ml 0.9 12.3%
    B 8 6.2pg/ml 0.7 10.8%
    C 8 8.77pg/ml 0.7 7.6%
    D 8 7.36pg/ml 0.4 6%
    Inter-assay
    Sample n Mean SD CV%
    A 9 7.1pg/ml 0.83 11.8%
    B 9 6.47pg/ml 0.72 11.1%
    C 9 8.76pg/ml 1.37 15.7%
    D 9 7.44pg/ml 1.2 16.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.31 pg/ml
  • Range
    0.31 pg/ml - 10 pg/ml
  • Recovery

    101 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 110 87% - 127%
    Serum 69 61% - 75%

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human high sensitivity IL-4 ELISA is to be used for the in-vitro quantitative determination interleukin-4 (IL-4) in human sera, plasmas, buffered solutions and cell culture media. The assay will recognize both natural and recombinant human IL-4. This kit is intended for research use only and is not to be used in diagnostic procedures.

    PRINCIPLE OF THE METHOD

    The IL-4 kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations and unknowns are pipetted into these wells.

    During the first incubation, the IL-4 antigen and a biotinylated monoclonal antibody specific for IL-4 are successivly incubated.

    After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotin-Tyramine reagent. Under the action of HRP, a biotin polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotin is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IL-4 present in the samples.

     

    Recognizes both natural and recombinant human IL4

    Range: 0.31 pg/ml - 10 pg/ml

    Sensitivity < 0.31 pg/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceIL4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL4. IL4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL4 encoding distinct isoforms have been reported.
  • Cellular localizationSecreted
    • Alternative names
        B cell growth factor 1B cell stimulatory factor 1 BCGF 1
        BCGF1Binetrakin BSF 1BSF1 IL 4Interleukin 4Lymphocyte stimulatory factor 1MGC79402Pitrakinra
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab46063 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      IL4 High Sensitivity Human ELISA Kit images

      • ELISA - Human High Sensitivity IL4 ELISA Kit (ab46063)
        ELISA - Human High Sensitivity IL4 ELISA Kit (ab46063)
        Typical IL-4 High sensitivity standard curve ranging from 0.31 to 10 pg/ml

      References for IL4 High Sensitivity Human ELISA Kit (ab46063)

      ab46063 has not yet been referenced specifically in any publications.

      Publishing research using ab46063 ? Please let us know so that we can cite the reference in this datasheet

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"