IL4 High Sensitivity Human ELISA Kit (ab46063)
- Product nameIL4 High Sensitivity Human ELISA KitSee all IL4 kits ...
Intra-assay Sample n Mean SD CV% A 8 7.43pg/ml 0.9 12.3% B 8 6.2pg/ml 0.7 10.8% C 8 8.77pg/ml 0.7 7.6% D 8 7.36pg/ml 0.4 6% Inter-assay Sample n Mean SD CV% A 9 7.1pg/ml 0.83 11.8% B 9 6.47pg/ml 0.72 11.1% C 9 8.76pg/ml 1.37 15.7% D 9 7.44pg/ml 1.2 16.1%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.31 pg/ml
- Range0.31 pg/ml - 10 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 87% - 127% Serum 69 61% - 75%
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
The Human high sensitivity IL-4 ELISA is to be used for the in-vitro quantitative determination interleukin-4 (IL-4) in human sera, plasmas, buffered solutions and cell culture media. The assay will recognize both natural and recombinant human IL-4. This kit is intended for research use only and is not to be used in diagnostic procedures.
PRINCIPLE OF THE METHOD
The IL-4 kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations and unknowns are pipetted into these wells.
During the first incubation, the IL-4 antigen and a biotinylated monoclonal antibody specific for IL-4 are successivly incubated.
After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotin-Tyramine reagent. Under the action of HRP, a biotin polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotin is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IL-4 present in the samples.
Recognizes both natural and recombinant human IL4
Range: 0.31 pg/ml - 10 pg/ml
Sensitivity < 0.31 pg/ml
- For research use only.
- The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
- Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.
- Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
- Do not pipette by mouth.
- The stop solution in this kit contains 9.8% sulphuric acid.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 200X Washing Buffer White 1 x 10ml Amplification Diluent Brown & blue spot 1 x 25ml Amplifier Yellow 1 x 200µl Biotinylated Antibody Diluent Red 1 x 7.5ml Chromogen TMB 1 x 11ml HRP Diluent Red 1 x 23ml IL4 Biotinylated anti-IL4 Antibody 1 x 0.4ml IL4 precoated microtiter plates 8 units IL4 Standard 2 vials Stop Reagent Black 1 x 11ml Streptavidin-HRP 2 x 5µl
- Research Areas
- B cell growth factor 1B cell stimulatory factor 1 BCGF 1
- BCGF1Binetrakin BSF 1BSF1 IL 4Interleukin 4Lymphocyte stimulatory factor 1MGC79402Pitrakinra
Our Abpromise guarantee covers the use of ab46063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IL4 High Sensitivity Human ELISA Kit images
Typical IL-4 High sensitivity standard curve ranging from 0.31 to 10 pg/ml
References for IL4 High Sensitivity Human ELISA Kit (ab46063)
ab46063 has not yet been referenced specifically in any publications.