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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Overview
Product name
Anti-Hippocalcin antibody
See all Hippocalcin products (3) ...
Description
Rabbit polyclonal to Hippocalcin
Tested applications
WB, IHC-P, IHC (PFA fixed), IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Rat Hippocalcin.
(Peptide available as ab25848.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: 1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent concentration.
Immunohistochemistry (PFA fixed): Use at a concentration of 0.2 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Hippocalcin is a high-affinity calcium-binding protein restricted to the CNS and most abundant in pyramidal cells of the hippocampal CA1 region. It belongs to the family of EF-hand-containing neuronal calcium sensor (NCS) proteins that possess a Ca2+/myristoyl switch allowing translocation to membranes in response to increased cytosolic [Ca2+]. Hippocalcin can regulate mixed lineage kinase 2, phospholipase D and the neuronal apoptosis inhibitory protein.
Cellular localization
Cell Membrane and Cytoplasmic
Alternative names
- BDR 2 antibody
- BDR2 antibody
- Calcium binding protein BDR2 antibody
see all
Database links
- Entrez Gene: 3208 Human
- Entrez Gene: 15444 Mouse
- Entrez Gene: 29177 Rat
- Omim: 142622 Human
- SwissProt: P84074 Human
- SwissProt: P84075 Mouse
- SwissProt: P84076 Rat
- Unigene: 11019 Rat
Anti-Hippocalcin antibody images:
Immunohistochemistry (PFA fixed) - Hippocalcin antibody (ab24560)
Immuofluorescent staining for Hippocalcin in the rat hippocampus using Rabbit polyclonal to Hippocalcin (ab24560). This figure is a montage of pictures acquired with a X10 objective and shows expected abundance of staining in parts of the hippocampus such as the CA1 and CA3 and an absence of staining (as expected) in the cortex and the corpus callosum. ab24560 was used at 1/1000 (0.2µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
Sophie Pezet, King`s college London, United Kingdom
Western blot - Hippocalcin antibody (ab24560)
All lanes : Anti-Hippocalcin antibody (ab24560) at 1 µg/ml
Lane 1 : Mouse Brain
Lane 2 : Day 0 Mouse Brain
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 14 kDa (possible cleavage fragment),45&52&100 kDa (possible cross reactivity).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hippocalcin antibody (ab24560)
Image courtesy of Human Protein Atlas
ab24560 staining Hippocalcin in human cerebellum. Paraffin embedded tissue was cut into 4µm sections and incubated with ab24560 (1/350 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab24560 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
References for Anti-Hippocalcin antibody (ab24560)
This product has been referenced in:
- Kathiresan Tet al. A protein interaction network for the large conductance Ca(2+)-activated K(+) channel in the mouse cochlea. Mol Cell Proteomics 8:1972-87 (2009).Read more (PubMed: 19423573) »
- Osheroff H & Hatten ME Gene expression profiling of preplate neurons destined for the subplate: genes involved in transcription, axon extension, neurotransmitter regulation, steroid hormone signaling, and neuronal survival. Cereb Cortex 19 Suppl 1:i126-34 (2009).Read more (PubMed: 19398467) »
See all 2 publications for this product
Publishing research using ab24560? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"