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Western blot - Histidase antibody (ab55232)

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Product Name
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Histidase antibody
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See all Histidase antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Histidase
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Immunogen
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Recombinant fragment: LAACQGIEFL RPLKTTTPLE KVYDLVRSVV RPWIKDRFMA PDIEAAHRLL LEQKVWEVAA PYIEKYRMEH IPESRPLSPT AFSLQFLHKK STKIPESEDL , corresponding to amino acids 558-658 of Human Histidase BLAST 'LAACQGIEFL RPLKTTTPLE KVYDLVRSVV RPWIKDRFMA PDIEAAHRLL LEQKVWEVAA PYIEKYRMEH IPESRPLSPT AFSLQFLHKK STKIPESEDL ' with or 
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Reacts with
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RecFrag
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Predicted to react
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Hu (Due to sequence homology)
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia, which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. The eukaryotic enzyme, histidase (L-histidine ammonia-lyase) is a particularly apt model for the study of the control of gene expression at the molecular level during differentiation and subsequent development of tissues and endocrine regulation.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histidase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histidase antibody images, references, products related to ab55232 and other tools.
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Histidase antibody images:
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Western blot - Histidase antibody (ab55232)
Western blot against tagged recombinant protein immunogen using ab55232 Histidase antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
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Search PubMed (MEDLINE) for references to Histidase
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Histidase antibody - more information
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Customer reviews (feedback) regarding Histidase antibody |
Customer FAQs regarding Histidase antibody |
Protocols for Histidase antibody |
Price and availability of products related to Histidase antibody |
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Histidase antibody - related products:
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Histidase antibody - other tools:
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Contact Abcam with a Technical Enquiry about Histidase antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"