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Western blot - Histidase antibody (ab83831)
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Product Name
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Histidase antibody
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See all Histidase antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Histidase
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Immunogen
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Synthetic peptide corresponding to a region within the N terminal amino acids 180-229 (INKLQELQV NLVRSHSSGV GKPLSPERCR MLLALRINVL AKGYSGISLE of Human Histidase (NP_002099)
BLAST 'INKLQELQVN LVRSHSSGVG KPLSPERCRM LLALRINVLA KGYSGISLET ' with  or 
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Reacts with
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1/1562500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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721_B cell lysate
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia, which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids.
The eukaryotic enzyme, histidase (L-histidine ammonia-lyase) is a particularly apt model for the study of the control of gene expression at the molecular level during differentiation and subsequent development of tissues and endocrine regulation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histidase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histidase antibody images, references, products related to ab83831 and other tools.
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Histidase antibody images:
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 Western blot - Histidase antibody (ab83831)
Histidase antibody (ab83831) at 1 µg/ml (in 5% skim milk / PBS buffer) + 10ug 721_B cell lysate
Secondary
HRP conjugated anti-Rabbit IgG diluted 1/50,000 - 1/100,000
Predicted band size : 73 kDa
Observed band size : 73 kDa
Gel concentration 12%
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Search PubMed (MEDLINE) for references to Histidase
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Histidase antibody - more information
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Customer reviews (feedback) regarding Histidase antibody |
Customer FAQs regarding Histidase antibody |
Protocols for Histidase antibody |
Price and availability of products related to Histidase antibody |
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Histidase antibody - related products:
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Histidase antibody - other tools:
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Contact Abcam with a Technical Enquiry about Histidase antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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