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Products:Neuroscience >> Neurotransmitter >> Biogenic Amines >> Histamine
Overview
Product name
Anti-Histidine decarboxylase antibody
See all Histidine decarboxylase products (2) ...
Description
Rabbit polyclonal to Histidine decarboxylase
Specificity
Detects both the 74 kDa full length and the 54 kDa active form of Histone decarboxylase.
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide surrounding amino acid 22 of Human Histone decarboxylase.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Histamine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21873469)
WB: Use a concentration of 1 - 4 µg/ml.Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).Can be blocked with Histidine decarboxylase peptide (ab55801).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Pathway
Amine and polyamine biosynthesis; histamine biosynthesis; histamine from L-histidine: step 1/1.
Sequence similarities
Belongs to the group II decarboxylase family.
Target information above from: UniProt accessionP19113
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DCHS_HUMAN antibody
- EC 4.1.1.22 antibody
- HDC antibody
- Histidine decarboxylase antibody
Database links
- Entrez Gene: 3067 Human
- Entrez Gene: 15186 Mouse
- Entrez Gene: 24443 Rat
- Omim: 142704 Human
- SwissProt: P19113 Human
- SwissProt: P23738 Mouse
- SwissProt: Q7TMW5 Mouse
- SwissProt: Q9QWU3 Mouse
see all
Anti-Histidine decarboxylase antibody images:
Western blot - Histidine decarboxylase antibody (ab37291)
Predicted band size : 74 kDa
Western blot analysis of HDC expression in rat fetal liver tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histidine decarboxylase antibody (ab37291)
IHC image of ab37291 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37291, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Histidine decarboxylase antibody (ab37291)
This product has been referenced in:
- Francis Het al. Inhibition of histidine decarboxylase ablates the autocrine tumorigenic effects of histamine in human cholangiocarcinoma. Gut : (2011). ICC/IF; Human.Read more (PubMed: 21873469) »
- Davis SCet al. Up-regulation of histidine decarboxylase expression and histamine content in B16F10 murine melanoma cells. Inflamm Res 60:55-61 (2011). WB, ICC/IF; Mouse.Read more (PubMed: 20640870) »
See all 4 publications for this product
Publishing research using ab37291? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"