Overview

  • Product nameHistoReveal (15ml)
  • General notesHistoReveal is a single stable solution, designed to unmask immunoreactive sites altered by fixation and/or the embedding process.

    The most commonly used tissue fixatives are aldehydes, such as formalin, which work by protein crosss-linking. Proper fixation of a specimen prevents antigen from being washed out and is a critical aspect of successful IHC staining. Cross-linking can mask antigens, preventing primary antibodies from binding, thus yielding weak staining or false negative results. Immunoglobulins are especially susceptible to formalin fixation.

    Treatment with a proteolytic enzyme is required to digest excess aldehyde linkages and expose the antigen. HistoReveal is a proteolytic enzyme solution that can be used in place of traditionally used enzyme pretreatments, such as pepsin or trypsin, and results in superior staining. Unlike other enzymes, HistoReveal is gentle on tissue and does not destroy morphology.

    Moreover HistoReveal requires only a brief incubation at room temperature. Performing antigen recovery with HistoReveal will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining.

    Digestion Procedure

    • Deparaffinize tissue sections and bring them into buffer.
    • Remove excess buffer from slides without letting tissue dry.
    • Depending on tissue section, add 1 or 2 drops of HistoReveal.
    • Incubate slides for 5 minutes at room temperature.
    • Wash slides with buffer and proceed with immunostaining.

Properties

    HistoReveal (15ml) images

    • Cytokeratin 20 being stained in colon tissue following antigen retrieval using HistoReveal.

    References for HistoReveal (15ml) (ab103720)

    ab103720 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.


    I have placed the protocol below. This product is to be used for antigen retrieval in place of pepsin or trypsin for enzymatic retrieval.

    PROCEDURE:

    1. Deparaffinize tissue sections and bring...

    Read More

    Thank you for confirming the details. The HistoReveal ab103720 use in IHC is based on enzymatic antigen retrieval method. As there is no step of heating the tissue sections so probably this could be product as per your requirements. However I ca...

    Read More

    Thank you for contacting us. Could you specify which target you would be interested in seeing with antibodies? I don’t think the antigen retrieval method will affect the target detection in IHC-IF. However if you are interested in extra...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"