• Product nameAnti-Histone H1 antibody [AE-4]See all Histone H1 primary antibodies ...
  • Description
    Mouse monoclonal [AE-4] to Histone H1
  • SpecificityHistone H1 itself is a highly conserved protein and is practically identical in sequence across most species. Given this, species cross-reactivity may be expected. In blots of rat brain homogenate a duplet is observed at 27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
  • Tested applicationsIHC-Fr, IHC-P, ELISA, WB, ICC, ICC/IF, Electron Microscopymore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Nuclei from myeloid leukamia biopsy cells.

  • Positive control
    • IHC-P: Human normal colon FFPE tissue sections.



Our Abpromise guarantee covers the use of ab71594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB 1/100.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution. PubMed: 21063722


  • FunctionMay play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
  • Tissue specificityOocyte-specific.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localizationCytoplasm. Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ221C16.2 antibody
    • dJ221C16.5 antibody
    • DmeH1 antibody
    • H1 0 histone antibody
    • H1 antibody
    • H1 histone family member 0 antibody
    • H1 histone family member 1 antibody
    • H1 histone family member 3 antibody
    • H1 histone family member 5 antibody
    • H1 histone family member N testis specific antibody
    • H1 histone family member O oocyte specific antibody
    • H1 histone family member X antibody
    • H1(0) antibody
    • H1.1 antibody
    • H1.2 antibody
    • H1.3 antibody
    • H1.4 antibody
    • H1.5 antibody
    • H10 antibody
    • H1A antibody
    • H1f0 antibody
    • H1F0 protein antibody
    • H1F1 antibody
    • H1F2 antibody
    • H1F3 antibody
    • H1F4 antibody
    • H1F5 antibody
    • H1FNT antibody
    • H1FOO antibody
    • H1FOO protein antibody
    • H1FOO_HUMAN antibody
    • H1FT antibody
    • H1FV antibody
    • H1FX antibody
    • H1oo antibody
    • H1t antibody
    • H1T2 antibody
    • H1X antibody
    • HANP1 antibody
    • His1 antibody
    • HisC antibody
    • HIST1 antibody
    • HIST1H1A antibody
    • HIST1H1B antibody
    • HIST1H1C antibody
    • HIST1H1D antibody
    • HIST1H1E antibody
    • HIST1H1T antibody
    • Histone 1 H1a antibody
    • Histone 1 H1b antibody
    • Histone 1 H1c antibody
    • Histone 1 H1d antibody
    • Histone 1 H1e antibody
    • Histone 1 H1t antibody
    • Histone antibody
    • Histone H1' antibody
    • Histone H1.0 antibody
    • Histone H1.1 antibody
    • Histone H1.2 antibody
    • Histone H1.3 antibody
    • Histone H1.4 antibody
    • Histone H1.5 antibody
    • Histone H1oo antibody
    • Histone H1x antibody
    • MGC116819 antibody
    • MGC126630 antibody
    • MGC126632 antibody
    • MGC126642 antibody
    • MGC138176 antibody
    • MGC138345 antibody
    • MGC15959 antibody
    • MGC3992 antibody
    • MGC50807 antibody
    • MGC5241 antibody
    • MGC8350 antibody
    • Oocyte specific histone H1 antibody
    • Oocyte specific linker histone H1 antibody
    • Oocyte-specific histone H1 antibody
    • Oocyte-specific linker histone H1 antibody
    • osH1 antibody
    • Testicular H1 histone antibody
    see all

Anti-Histone H1 antibody [AE-4] images

  • IHC image of ab71594 staining Histone H1 in Human normal colon formalin fixed paraffin embedded tissue* sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71594, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab71594 staining Histone H1 in HeLa cells by Immunocytochemistry/ Electron Microscopy.
    Cells were frozen under high pressure. Samples were incubated with ab71594 at 5µg/ml in 1% serum, 1% BSA, PBS. The secondary used was a donkey anti-mouse conjugated to 10nM gold, 1/30 dilution.

References for Anti-Histone H1 antibody [AE-4] (ab71594)

This product has been referenced in:
  • Kagiwada S  et al. Replication-coupled passive DNA demethylation for the erasure of genome imprints in mice. EMBO J 32:340-53 (2013). Read more (PubMed: 23241950) »
  • Singh SK  et al. GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy. Nat Commun 4:1830 (2013). Human . Read more (PubMed: 23652018) »

See all 6 Publications for this product

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

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Thank you for your reply.

Yes, I did contact the supplier of this antibody and I have forwarded you all of the information that they provided to me. As for the immunogen, the datasheet states that "Nuclei from myeloid leukamia biopsy cel...

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Thank you for your patience as I tried to find out more information regarding ab71594.
We have only tested this antibody on Histone H1. From the other publications, this antibody has been tested with HUVECs, HeLa cells and Neonatal cord blood. In ...

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Thank you for your inquiry regarding ab71594, anti-Histone H1. .I have looked into your inquiry regarding the specificity of this antibody. From the information that I have, this antibody does not have tissue specificity. I have e-mailedthe lab in the ...

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Thank you for contacting us.

H1.2 specific antibodies,
ab4086; http://www.abcam.com/Histone-H1-2-antibody-ChIP-Grade-ab4086.html. (we however haven't tested the specificity to other forms)
ab17677; http://www.abcam.com/Histone-H1-2-...

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Thank you for contacting us.

ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

ab83058; the sequence used is from P07305 http://...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (HeLa cells)
Specification HeLa cells
Fixative high-pressure freezing
Permeabilization No

Dr. Margarita Sobol

Verified customer

Submitted Jan 25 2011