Overview

  • Product nameAnti-Histone H1 antibody [EPR6537]See all Histone H1 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR6537] to Histone H1
  • Tested applicationsWB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of Human Histone H1.

  • Positive control
    • WB: BxPC3 and fetal Human lung lysates IHC-P: Human kidney tissue ICC/IF: A431 cells
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab125027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionMay play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
    • Tissue specificityOocyte-specific.
    • Sequence similaritiesBelongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.
    • Cellular localizationCytoplasm. Nucleus. Chromosome.
    • Target information above from: UniProt accession Q8IZA3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • dJ221C16.2 antibody
      • dJ221C16.5 antibody
      • DmeH1 antibody
      • H1 0 histone antibody
      • H1 antibody
      • H1 histone family member 0 antibody
      • H1 histone family member 1 antibody
      • H1 histone family member 3 antibody
      • H1 histone family member 5 antibody
      • H1 histone family member N testis specific antibody
      • H1 histone family member O oocyte specific antibody
      • H1 histone family member X antibody
      • H1(0) antibody
      • H1.1 antibody
      • H1.2 antibody
      • H1.3 antibody
      • H1.4 antibody
      • H1.5 antibody
      • H10 antibody
      • H1A antibody
      • H1f0 antibody
      • H1F0 protein antibody
      • H1F1 antibody
      • H1F2 antibody
      • H1F3 antibody
      • H1F4 antibody
      • H1F5 antibody
      • H1FNT antibody
      • H1FOO antibody
      • H1FOO protein antibody
      • H1FOO_HUMAN antibody
      • H1FT antibody
      • H1FV antibody
      • H1FX antibody
      • H1oo antibody
      • H1t antibody
      • H1T2 antibody
      • H1X antibody
      • HANP1 antibody
      • His1 antibody
      • HisC antibody
      • HIST1 antibody
      • HIST1H1A antibody
      • HIST1H1B antibody
      • HIST1H1C antibody
      • HIST1H1D antibody
      • HIST1H1E antibody
      • HIST1H1T antibody
      • Histone 1 H1a antibody
      • Histone 1 H1b antibody
      • Histone 1 H1c antibody
      • Histone 1 H1d antibody
      • Histone 1 H1e antibody
      • Histone 1 H1t antibody
      • Histone antibody
      • Histone H1.0 antibody
      • Histone H1.1 antibody
      • Histone H1.2 antibody
      • Histone H1.3 antibody
      • Histone H1.4 antibody
      • Histone H1.5 antibody
      • Histone H1oo antibody
      • Histone H1x antibody
      • MGC116819 antibody
      • MGC126630 antibody
      • MGC126632 antibody
      • MGC126642 antibody
      • MGC138176 antibody
      • MGC138345 antibody
      • MGC15959 antibody
      • MGC3992 antibody
      • MGC50807 antibody
      • MGC5241 antibody
      • MGC8350 antibody
      • Oocyte specific histone H1 antibody
      • Oocyte specific linker histone H1 antibody
      • Oocyte-specific histone H1 antibody
      • Oocyte-specific linker histone H1 antibody
      • osH1 antibody
      • Testicular H1 histone antibody
      see all

    Anti-Histone H1 antibody [EPR6537] images

    • All lanes : Anti-Histone H1 antibody [EPR6537] (ab125027) at 1/1000 dilution

      Lane 1 : BxPC3 lysates
      Lane 2 : Human fetal lung lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 21 kDa
    • ab125027, at a 1/100 dilution, staining Histone H1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab125027, at a 1/100 dilution, staining Histone H1 in A431 cells by Immunofluorescence.

    References for Anti-Histone H1 antibody [EPR6537] (ab125027)

    This product has been referenced in:
    • Kong X  et al. GSK3ß is a checkpoint for TNF-a-mediated impaired osteogenic differentiation of mesenchymal stem cells in inflammatory microenvironments. Biochim Biophys Acta 1830:5119-29 (2013). Read more (PubMed: 23911749) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us.

    H1.2 specific antibodies,
    ab4086; http://www.abcam.com/Histone-H1-2-antibody-ChIP-Grade-ab4086.html. (we however haven't tested the specificity to other forms)
    ab17677; http://www.abcam.com/Histone-H1-2-...

    Read More

    Thank you for contacting us.

    ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

    ab83058; the sequence used is from P07305 http://...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"