Anti-Histone H1 (phospho T17) antibody (ab61060)

Overview

• Product nameAnti-Histone H1 (phospho T17) antibodySee all Histone H1 primary antibodies ...
• Description
  Rabbit polyclonal to Histone H1 (phospho T17)
• Specificityab61060 detects endogenous levels of Histone H1 only when phosphorylated at threonine 17.
• Tested applicationsICC/IF, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic phosphopeptide derived from human Histone H1 around the phosphorylation site of threonine 17 (EKT^pPV).

• Positive controlHuvEc cells, treated with serum (20%, 30mins).

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab61060 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Histones
  • H1
  • Phosphorylated

Applications

Target

• FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher order structures.
• Sequence similaritiesBelongs to the histone H1/H5 family.
  Contains 1 H15 (linker histone H1/H5 globular) domain.
• Post-translational
  modificationsAcetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
• Cellular localizationNucleus. Chromosome.

Target information above from: UniProt accession P10412 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3008 Human
  • Entrez Gene: 50709 Mouse
  • Omim: 142711 Human
  • SwissProt: P16401 Human
  • SwissProt: P10412 Human
  • SwissProt: P43276 Mouse
  • SwissProt: P43274 Mouse
  • Unigene: 131956 Human

  • Unigene: 248133 Human
  • Unigene: 221314 Mouse
  • Unigene: 170587 Mouse

  see all

• Alternative names
  H1 histone family antibodyH14_HUMAN antibodyH1F4 antibody

  H1F5 antibodyHist1h1e antibodyHistone H1.4 antibodyHistone H1a antibodyHistone H1b antibody

  see all

Anti-Histone H1 (phospho T17) antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Histone H1 (phospho T17) antibody (ab61060)
  Immunofluorescence analysis, using Histone H1 (phospho T17) antibody (ab61060) at 1/500 - 1/1000 dilution, of HuvEc cells with serum treatment (20%, 30mins)(left panel) or without serum treatment (right panel).