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Product name
Anti-Histone H1 (phospho) antibody
Description
Rabbit polyclonal to Histone H1 (phospho)
Specificity
Recognizes phosphorylated histone H1
Tested applications
Flow Cyt, WB, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Hyper-phosphorylated isoform of tetrahymena Histone H1
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.035% Sodium Azide
Constituents: 30% Glycerol, 0.7M Tris glycine, 0.115M Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Histones >> H1 >> Phosphorylated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa.Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml).
ICC: Use a concentration of 5 - 10 µg/ml.
Target
Relevance
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction.
Cellular localization
Nuclear
Alternative names
- H1 0 antibody
- H1 histone family member 0 antibody
- H1(0) antibody
see all
Database links
- Entrez Gene: 3005 Human
- Omim: 142708 Human
- SwissProt: P07305 Human
- Unigene: 226117 Human
Anti-Histone H1 (phospho) antibody images:
Immunofluorescence - Histone H1 (phospho) antibody (ab4270)
Rabbit polyclonal to Histone H1 (phospho) (1/100).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab4270 (green). The DNA stained with DAPI is shown in red. (100x magnification).
Dr Michael Hendzels Laboratory
Western blot - Histone H1 (phospho) antibody (ab4270)
All lanes : Anti-Histone H1 (phospho) antibody (ab4270) at 2 µg/ml
Lane 1 : Colcemid treated HeLa cell lysate
Lane 2 : Untreated HeLa cell lysate
Secondary
Lane 1 : Goat anti-rabbit
secondary antibody labeled with HRP
Lane 2 : Goat anti-rabbit
secondary antibody labeled with HRP.
developed using the ECL technique
References for Anti-Histone H1 (phospho) antibody (ab4270)
This product has been referenced in:
- Chien WW et al. S-phase lengthening induced by p16(INK4a) overexpression in malignant cells with wild-type pRb and p53. Cell Cycle 9: (2010). WB; Human.Read more (PubMed: 20703084) »
- Ingemarsdotter CK et al. Low-dose paclitaxel synergizes with oncolytic adenoviruses via mitotic slippage and apoptosis in ovarian cancer. Oncogene 29:6051-63 (2010). WB; Human.Read more (PubMed: 20729921) »
See all 3 publications for this product
Publishing research using ab4270? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"