Anti-Histone H1.0 antibody [27] (ab11080)
Key features and details
- Mouse monoclonal [27] to Histone H1.0
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Histone H1.0 antibody [27]
See all Histone H1.0 primary antibodies -
Description
Mouse monoclonal [27] to Histone H1.0 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, VertebrataDoes not react with: Bird -
Immunogen
Full length native protein (purified) corresponding to Cow Histone H1.0.
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Epitope
This antibody recognises an epitope within aa24-30. Proline 26, which is responsible for a bend in this region, plays an important role in the recognition. See Gorka et al. 1998 for more information. -
Positive control
- WB: A431, MCF7 and HeLa cell lysates; Histone H1.0 Human Recombinant Protein. IHC-P: Human colon and pancreas adenocarcinoma tissues.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
27 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 20 kDa).
Linker histones run at about 30kD even though the predicted size is about 20kD. |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 20 kDa). Linker histones run at about 30kD even though the predicted size is about 20kD. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Post-translational
modificationsPhosphorylated on Ser-17 in RNA edited version. -
Cellular localization
Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 3005 Human
- Entrez Gene: 14958 Mouse
- Entrez Gene: 24437 Rat
- Omim: 142708 Human
- SwissProt: Q0IIJ2 Cow
- SwissProt: P07305 Human
- SwissProt: P10922 Mouse
- SwissProt: P43278 Rat
see all -
Alternative names
- H1 histone family member 0 antibody
- H1(0) antibody
- H10 antibody
see all
Images
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All lanes : Anti-Histone H1.0 antibody [27] (ab11080) at 1/500 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : H1F0 knockout A431 cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab11080 observed at 30 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab11080 was shown to react with Histone H1.0 in wild-type A431 cells in Western blot with loss of signal observed in H1F0 knockout sample. Wild-type A431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab11080 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-Histone H1.0 antibody [27] (ab11080) at 1/500 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Histone H1.0 Human Recombinant Protein
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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IHC image of Histone H1.0 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11080, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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Datasheet download
References (9)
ab11080 has been referenced in 9 publications.
- Yan X et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB ; Rat . PubMed: 27611779
- Fu G et al. Mouse oocytes and early embryos express multiple histone H1 subtypes. Biol Reprod 68:1569-76 (2003). PubMed: 12606334
- Khochbin S Histone H1 diversity: bridging regulatory signals to linker histone function. Gene 271:1-12 (2001). PubMed: 11410360
- Adenot PG et al. Somatic linker histone H1 is present throughout mouse embryogenesis and is not replaced by variant H1 degrees. J Cell Sci 113 ( Pt 16):2897-907 (2000). PubMed: 10910774
- Grunwald D et al. In situ analysis of chromatin proteins during development and cell differentiation using flow cytometry. Methods Mol Biol 119:443-54 (1999). PubMed: 10804531
- Gorka C et al. Differential recognition of histone H10 by monoclonal antibodies during cell differentiation and the arrest of cell proliferation. J Biol Chem 273:1208-15 (1998). PubMed: 9422788
- Gorka C et al. Variation of H1(0) content throughout the cell cycle in regenerating rat liver. Exp Cell Res 217:528-33 (1995). PubMed: 7698253
- Grunwald D et al. Accumulation of histone H1(0) during early Xenopus laevis development. Exp Cell Res 218:586-95 (1995). PubMed: 7796895
- Seigneurin D et al. Developmentally regulated chromatin acetylation and histone H1(0) accumulation. Int J Dev Biol 39:597-603 (1995). PubMed: 8619958