Recombinant Anti-Histone H1.3 antibody [EPR12683] (ab183736)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12683] to Histone H1.3
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Histone H1.3 antibody [EPR12683]
See all Histone H1.3 primary antibodies -
Description
Rabbit monoclonal [EPR12683] to Histone H1.3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: ChIP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- T47-D, HeLa & Jurkat whole cell lysate (ab7899), Human cervix carcinoma tissue, HeLa & T47-D cells Flow: Hela ICC: T47-D cells, Hela IHC: Human cervix carcinoma tissue IP: HeLa
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12683 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/500.
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IP |
1/30.
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Notes |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
IP
1/30. |
Target
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Function
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
Sequence similarities
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3007 Human
- GenBank: NP_005320 Human
- Omim: 142210 Human
- SwissProt: P16402 Human
- Unigene: 136857 Human
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Alternative names
- H1 histone family member 3 antibody
- H1.3 antibody
- H13_HUMAN antibody
see all
Images
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All lanes : Anti-Histone H1.3 antibody [EPR12683] (ab183736) at 1/5000 dilution
Lane 1 : T-47D cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDaBlocking buffer: 5% NFDM/TBST
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Intracellular Flow Cytometry analysis of HeLa cells usingab183736 at 1/10 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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Immunofluorescence analysis of T47-D cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi
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Immunofluorescence analysis of HeLa cells labeling Histone H1.3 with ab183736 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Histone H1.3 with ab183736 at 1/1000 dilution. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Histone H1.3 was immunoprecipitated from 0.35 mg of HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate with ab183736 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183736 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Hela whole cell lysate 10 μg (Input).
Lane 2: ab183736 IP in Hela whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183736 in Hela whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab183736 has been referenced in 1 publication.
- Yang W et al. Downregulation of lncRNA APCDD1L-AS1 due to DNA hypermethylation and loss of VHL protein expression promotes the progression of clear cell renal cell carcinoma. Int J Biol Sci 18:2583-2596 (2022). PubMed: 35414787