Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
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ab46666 |
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ab18263 has been referenced in 3 publications.
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All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab18263)
Lane 1 : Butyrate-treated HeLa histones
Lane 2 : 15-day embryo chicken erythrocyte histones
Lane 3 : Recombinant H2A.Z
Lane 4 : Butyrate-treated HeLa histones
Lane 5 : 15-day embryo chicken erythrocyte histones
Lane 6 : Recombinant H2A.Z
Predicted band size : 13 kDa
Observed band size : 13 kDa
Prof Colyn Crane-Robinson
All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab18263)
Lane 1 : Butyrate-treated HeLa histones
Lane 2 : Ix3 extract
Lane 3 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.
Lane 4 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.
Lane 5 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.
Lane 6 : Butyrate-treated HeLa histones
Lane 7 : Ix3 extract
Lane 8 : Nucleosomes from 10-day chicken brain tissue. ChIP Input.
Lane 9 : Nucleosomes from 10-day chicken brain tissue. ChIP Unbound.
Lane 10 : Nucleosomes from 10-day chicken brain tissue. ChIP Bound.
Predicted band size : 13 kDa
Observed band size : 13 kDa
Nucleosomes from 10-day chicken brain tissue were used in ChIP experiments. An acetic acid / urea / Triton gel was run and the corresponding western blot using ab18263 was used to compare proteins in the Bound chromatin fraction with those of the Input and Unbound fractions. In lane 10 there is a strong band corresponding to a hyper-acetylated H2A.Z.
Prof Colyn Crane-Robinson
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