This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Histone H2A (mono methyl K127) antibody (ab12950)

Overview

  • Product nameAnti-Histone H2A (mono methyl K127) antibodySee all Histone H2A primary antibodies ...
  • Description
    Rabbit polyclonal to Histone H2A (mono methyl K127)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • SpecificityELISA data demonstrates that ab12950 specifically recognizes mono-methyl Histone H2A K127. However, mono-methyl histone H2A K127 is a putative modification and is not detectable in HeLa, HeLa nuclear or calf thymus histone extracts using WB analysis. This may be due to low levels of this modification in these cell lines. ab12950 weakly recognizes acetyl histone H2A K127 in HeLa extracts in WB and recognizes recombinant histone H3 at high concentration levels in WB.
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H2A.

    (Peptide available as ab12952.)

Properties

Applications

  • Application notesThis antibody gave a positive result in ELISA against the immunizing peptide (ab12952). It gave a negative result in ELISA against the non-modified equivalent peptide (ab13202). This indicates that it is specific for the modified peptide.
    See figure below.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • Sequence similaritiesBelongs to the histone H2A family.
    • Post-translational
      modifications
      The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
      Deiminated on Arg-4 in granulocytes upon calcium entry.
      Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
      Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
      Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    • Cellular localizationNucleus. Chromosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • FO108 antibody
      • H2A antibody
      • H2A1B_HUMAN antibody
      • H4 histone family 2 antibody
      • H4 histone family member N antibody
      • H4F2 antibody
      • H4FN antibody
      • HIST1H2AE antibody
      • HistH2A antibody
      • Histone H2A type 1-B/E antibody
      • Histone H2A.2 antibody
      • Histone H2A/a antibody
      • Histone H2A/m antibody
      • Novel histone antibody
      see all

    Anti-Histone H2A (mono methyl K127) antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • ELISA eluate (red, blue) and flow through (green) using immunizing peptide (ab12952). Eluate (orange) and flow through (grey) using un-modified peptide (ab13202).

      This demonstrates that ab12950 specifically recognises mono-methyl K127 compared to the unmodified peptide.

    References for Anti-Histone H2A (mono methyl K127) antibody (ab12950)

    ab12950 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Jun 13 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"