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Histone H2A peptide - mono methyl K127 (ab12952)

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Overview

  • Product nameHistone H2A peptide - mono methyl K127See all Histone H2A proteins and peptides ...
  • Protein descriptionSynthetic peptide derived from within residues 100 to the C-terminus of Human Histone H2A. (Note: the amino acid sequence is proprietary) This peptide was used as an immunogen for ab12950 .
  • Protein length129 amino acids

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferInformation available upon request.
  • Concentration information loading...
  • Research Areas

    • Applications

  • Application notesThis peptide can be used with studies using ab12950 and ab13202.

    • Protein info

    • Alternative names
        FO108H2AH2A1B_HUMAN
        H4 histone family 2H4 histone family member NH4F2H4FNHIST1H2AEHistH2AHistone H2A type 1-B/EHistone H2A.2Histone H2A/aHistone H2A/m
      see all
  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H2A family.
  • Post-translational
    modifications    The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • Cellular localizationNucleus. Chromosome.

    • Target information above from: UniProt accession P04908 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Histone H2A peptide - mono methyl K127 images

    • ELISA - Histone H2A (mono methyl K127) antibody (ab12950)
      ELISA - Histone H2A (mono methyl K127) antibody (ab12950)
      ELISA eluate (red, blue) and flow through (green) using immunizing peptide (ab12952). Eluate (orange) and flow through (grey) using un-modified peptide (ab13202).

      This demonstrates that ab12950 specifically recognises mono-methyl K127 compared to the unmodified peptide.

    References for Histone H2A peptide - mono methyl K127 (ab12952)

    ab12952 has not yet been referenced specifically in any publications.

    Publishing research using ab12952 ? Please let us know so that we can cite the reference in this datasheet

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