Anti-Histone H2A.Z antibody [4A4] (ab80150)
Key features and details
- Rat monoclonal [4A4] to Histone H2A.Z
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG2b
Overview
-
Product name
Anti-Histone H2A.Z antibody [4A4]
See all Histone H2A.Z primary antibodies -
Description
Rat monoclonal [4A4] to Histone H2A.Z -
Host species
Rat -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Mouse, Rat, Cow, Human, Pig, Xenopus laevis, Zebrafish, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Histone H2A.Z.
-
Positive control
- This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
4A4 -
Isotype
IgG2b -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa). |
Target
-
Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. -
Sequence similarities
Belongs to the histone H2A family. -
Post-translational
modificationsMonoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
-
Database links
- Entrez Gene: 287016 Cow
- Entrez Gene: 3015 Human
- Entrez Gene: 51788 Mouse
- Entrez Gene: 58940 Rat
- Omim: 142763 Human
- SwissProt: P0C0S4 Cow
- SwissProt: P0C0S5 Human
- SwissProt: P0C0S6 Mouse
see all -
Alternative names
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
see all
Images
-
All lanes : Anti-Histone H2A.Z antibody [4A4] (ab80150) at 1 µg/ml
Lane 1 : Histone H2A.Z recombinant protein
Lane 2 : Histone H2A recombinant protein
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab80150 has not yet been referenced specifically in any publications.