![Western blot - Histone H2B antibody [EP858Y] (ab40883) image](#ab40883a.gif)
![Immunohistochemistry (Paraffin-embedded sections) - Histone H2B (acetyl K12) antibody [EP858Y] (ab40883) image](#ab40883b.jpg)
![Immunocytochemistry/ Immunofluorescence - Histone H2B (acetyl K12) antibody [EP858Y] (ab40883) image](#ab40883c.jpg)
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Product name
Anti-Histone H2B (acetyl K12) antibody [EP858Y]
See all Histone H2B products (11) ...
Description
Rabbit monoclonal [EP858Y] to Histone H2B (acetyl K12)
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic acetylated peptide corresponding to residues surrounding Lys12 of Histone H2B.
Positive control
HeLa cells, Human colon carcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Primary antibody notes
This antibody only detects Histone H2B acetylated on Lysine 12.
Clonality
Monoclonal
Clone number
EP858Y
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Histones >> H2B >> Acetylated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.
WB: 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
Is unsuitable for FACS or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similarities
Belongs to the histone H2B family.
Post-translational
modifications
Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionQ5QNW6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H2B 1A antibody
- H2B antibody
- H2B histone family antibody
see all
Database links
- Entrez Gene: 54145 Human
- Entrez Gene: 440689 Human
- SwissProt: Q5QNW6 Human
- Unigene: 632451 Human
Anti-Histone H2B (acetyl K12) antibody [EP858Y] images:
Western blot - Histone H2B antibody [EP858Y] (ab40883)
![Western blot - Histone H2B antibody [EP858Y] (ab40883)](/ps/datasheet/Images/40/ab40883/ab40883a.gif)
Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883) at 1/2000 dilution + C6+TSA cell lysate at 10 µg
Predicted band size : 14 kDa
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
![Immunohistochemistry (Paraffin-embedded sections) - Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)](/ps/datasheet/Images/40/ab40883/ab40883b.jpg)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B ab40883 at a 1:250 dilution.
Immunocytochemistry/ Immunofluorescence - Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
![Immunocytochemistry/ Immunofluorescence - Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)](/ps/datasheet/Images/40/ab40883/ab40883c.jpg)
ab40883 (1:250) staining of HeLa cells by immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
![Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)](/ps/datasheet/images/40/ab40883/Histone-H2B-Primary-antibodies-ab40883-3.jpg)
ab40883 (1/500) staining Histone H2B (acetyl K12) in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised using 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Image courtesy of an abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
References for Anti-Histone H2B (acetyl K12) antibody [EP858Y] (ab40883)
ab40883 has not yet been referenced specifically in any publications.
Publishing research using ab40883? Please let us know so that we can cite the reference in this datasheet
![Anti-Histone H2B (acetyl K12) antibody [EP858Y] for Immunocytochemistry/ Immunofluorescence in Human (40883)](/ps/datasheet/images/40/ab40883/Histone-H2B-Primary-antibodies-ab40883-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"