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Product name
Anti-Histone H2B (acetyl) antibody - ChIP Grade
See all Histone H2B products (11) ...
Description
Rabbit polyclonal to Histone H2B (acetyl) - ChIP Grade
Specificity
This antibody recognises acetylated Lys 12 and/or Lysine 15 of Histone H2B.
Tested applications
ChIP, ELISA, IF, IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Toxoplasma gondii
Immunogen
Synthetic peptide: APKKAcGSKAcKAVYC, corresponding to amino acids 9-20 of Histone H2B.
APKKAcGSKAcKAVYC
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.02% sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Histones >> H2B >> Acetylated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
IF
IP
Application notes
ChIP: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
ELISA: 1/400
Western Blotting 1/400
Immunofluorescence microscopy 1/200
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similarities
Belongs to the histone H2B family.
Post-translational
modifications
Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionQ5QNW6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- H2B 1A antibody
- H2B antibody
- H2B histone family antibody
see all
Database links
- Entrez Gene: 54145 Human
- Entrez Gene: 440689 Human
- Entrez Gene: 104021 Mouse
- Entrez Gene: 64647 Rat
- SwissProt: Q5QNW6 Human
- SwissProt: Q00729 Rat
- Unigene: 632451 Human
Anti-Histone H2B (acetyl) antibody - ChIP Grade images:
Immunocytochemistry/ Immunofluorescence - Histone H2B (acetyl) antibody - ChIP Grade (ab1759)
ab1759 at a dilution of 1/200 staining the Female lymphoblastoid cell line, GM12616, by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected with a polyclonal goat anti-rabbit FITC.
The DAPI counterstain is shown pseudocoloured as white in (b). A pale staining chromosome could be identified based on the low level of histone H2B acetylation of the inactive X chromosome. An enlargement of the pale staining chromosome is shown with DAPI pseudocoloured as red for contrast against the antibody (green).
Western blot - Histone H2B (acetyl) antibody - ChIP Grade (ab1759)
ab1759 used in Western Blot.FR235222 treatment specifically affects the acetylation status of histone H4 but not the acetylation levels of H3 and H2B. Extracellular T. gondii parasites (RH WT, R20D9 [TgHDAC3T99A], and M3135E11 [TgHDAC3T99I]) were treated with the indicated concentrations of FR235222 for 4 hours and lysed. 50µg of total cell lysates were analyzed by Immunoblotting with an anti-AcH4, anti-AcH2B (ab1759 at a 1/1000 dilution), anti-H3 and anti–alpha-tubulin antibodies.
Image from Bougdour A et al, J Exp Med 206:953-66 (2009), Fig S2.
References for Anti-Histone H2B (acetyl) antibody - ChIP Grade (ab1759)
This product has been referenced in:
- Bougdour A et al. Drug inhibition of HDAC3 and epigenetic control of differentiation in Apicomplexa parasites. J Exp Med 206:953-66 (2009). WB; Toxoplasma gondii.Read more (PubMed: 19349466) »
- Koshibu K et al. Protein phosphatase 1 regulates the histone code for long-term memory. J Neurosci 29:13079-89 (2009). WB; Mouse.Read more (PubMed: 19828821) »
See all 5 publications for this product
Publishing research using ab1759? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"