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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab23913? Please let us know so that we can cite the reference in this datasheet
ab23913 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Histone H2B (testis specific) antibody (ab23913)
All lanes : Anti-Histone H2B (testis specific) antibody (ab23913) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg/ml with
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg with
Lane 4 : HeLa Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 17 kDa
ab23913 specifically recognises mouse Histone H2B (lane1). The signal is efficiently blocked using the immunizing peptide (lane2) but not the corresponding general human H2B peptide (lane3). This indicates that ab23913 specifically recognises testis specific H2B. To support this ab23913 does not recognise human Histone H2B in HeLa whole cell lysates (lane4).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2B (testis specific) antibody (ab23913)
Image courtesy of Human Protein Atlas
ab23913 staining Histone H2B (testis specific) in human tesis tissue, showing a distinct and strong staining pattern within the seminiferous tubules. Paraffin embedded human testis was incubated with ab3167 (1/3000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab23913 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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