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I am trying to find out if abcam will also provide hsitones H2A, H3,H1t (testis specific). Yours is the only firm which is able to provide H2B(testis specific). please mail me this info about the availability of the other testis specific antibodies. Also if ab32913 will react with rat testis antigen also. |
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ANSWER: |
Thank you for your enquiry. Currently we only have a H2B testis specific antibody. It has been tested and is guaranteed to work in mouse cells. We have antibodies against histones and histone modifications. However, this is the only testis specific antibody. I have performed an alignment and I can tell you that there is only a single residue change in the region where the immunogen was derived. Please see the link below; http://www.ebi.ac.uk/cgi-bin/jobresults/clustalw/clustalw-20070216-11383201.aln I hope this information helps, please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Histone H2B (testis specific) antibody (ab23913) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg/ml with
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg with
Lane 4 : HeLa Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 17 kDa
ab23913 specifically recognises mouse Histone H2B (lane1). The signal is efficiently blocked using the immunizing peptide (lane2) but not the corresponding general human H2B peptide (lane3). This indicates that ab23913 specifically recognises testis specific H2B. To support this ab23913 does not recognise human Histone H2B in HeLa whole cell lysates (lane4).
Image courtesy of Human Protein Atlas
ab23913 staining Histone H2B (testis specific) in human tesis tissue, showing a distinct and strong staining pattern within the seminiferous tubules. Paraffin embedded human testis was incubated with ab3167 (1/3000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab23913 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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