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Products:Epigenetics and Nuclear Signaling >> Histones >> Variants
Overview
Product name
Anti-Histone H2B (testis specific) antibody
Description
Rabbit polyclonal to Histone H2B (testis specific)
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H2B (testis specific).
Positive control
Testis Tissue Lysate (Mouse)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Histones >> H2B >> Unmodified
Epigenetics and Nuclear Signaling >> Histones >> Variants
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/3000Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).Can be blocked with Histone H2B (testis specific) peptide (ab24027).
Target
Relevance
In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved. Histone H2B testis specific is a variant of H2B expressed solely in the testis(SwissProt:Q96A08) and differs by the addition of three potential phosphorylation sites (serine 12, threonine 23 and threonine 34) and the repositionoing of 2 others (serine 5 and serine 6), resulting in a different phosphorylation map of the N-terminal tail.
Cellular localization
Nuclear
Alternative names
- HIST1H2BA antibody
- Testis-specific histone H2B antibody
- bA317E16.3 antibody
see all
Database links
- Entrez Gene: 255626 Human
- Entrez Gene: 319177 Mouse
- Omim: 609904 Human
- SwissProt: Q6NZA0 Human
- SwissProt: Q6NZA1 Human
- SwissProt: Q96A08 Human
- SwissProt: P70696 Mouse
- Unigene: 371887 Human
see all
Anti-Histone H2B (testis specific) antibody images:
Western blot - Histone H2B (testis specific) antibody (ab23913)
All lanes : Anti-Histone H2B (testis specific) antibody (ab23913) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg
Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg/ml with Histone H2B (testis specific) peptide (ab24027) at 1 µg/ml
Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) at 1 µg with Histone H2B peptide (ab13336) at 1 µg/ml
Lane 4 : HeLa Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 17 kDa
ab23913 specifically recognises mouse Histone H2B (lane1). The signal is efficiently blocked using the immunizing peptide (lane2) but not the corresponding general human H2B peptide (lane3). This indicates that ab23913 specifically recognises testis specific H2B. To support this ab23913 does not recognise human Histone H2B in HeLa whole cell lysates (lane4).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2B (testis specific) antibody (ab23913)
Image courtesy of Human Protein Atlas
ab23913 staining Histone H2B (testis specific) in human tesis tissue, showing a distinct and strong staining pattern within the seminiferous tubules. Paraffin embedded human testis was incubated with ab3167 (1/3000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab23913 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
.References for Anti-Histone H2B (testis specific) antibody (ab23913)
ab23913 has not yet been referenced specifically in any publications.
Publishing research using ab23913? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"