Overview

  • Product nameAnti-Histone H3 - HRP antibody (HRP)
  • Description
    Rabbit polyclonal to Histone H3 - HRP (HRP)
  • ConjugationHRP
  • Conjugation notesThis antibody has been conjugated with HRP. The concentration of the original antibody was 0.5 mg/ml, following conjugation the concentration observed will be higher.
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster)
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H3.

    (Peptide available as ab12149.)

  • Positive control
    • This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Yeast S.cerevisiae Whole Cell Lysate Drosophila Embryo Nuclear Extract IHC-P: Human skin FFPE tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab21054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 15.4 kDa).Can be blocked with Human Histone H3 peptide (ab12149).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • H3 histone antibody
    • H3 histone family member E pseudogene antibody
    • H3.3A antibody
    • H3F3 antibody
    • H3F3A antibody
    • HIST3H3 antibody
    • Histone H3 3 pseudogene antibody
    see all

Anti-Histone H3 - HRP antibody (HRP) images

  • IHC image of Histone H3 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21054, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Histone H3 - HRP antibody (HRP) (ab21054) at 1/5000 dilution

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179) at 10 µg
    Lane 2 : Yeast S.cerevisiae Whole Cell Lysate at 10 µg
    Lane 3 : Drosophila Embryo Nuclear Extract at 20 µg


    Performed under reducing conditions.

    Predicted band size : 15.4 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

References for Anti-Histone H3 - HRP antibody (HRP) (ab21054)

This product has been referenced in:
  • Wei W  et al. Soluble Frizzled-7 receptor inhibits Wnt signaling and sensitizes hepatocellular carcinoma cells towards doxorubicin. Mol Cancer 10:16 (2011). WB ; Human . Read more (PubMed: 21314951) »
  • Hiragami-Hamada K  et al. N-terminal phosphorylation of HP1{alpha} promotes its chromatin binding. Mol Cell Biol 31:1186-200 (2011). Read more (PubMed: 21245376) »

See all 5 Publications for this product

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