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Anti-Histone H3 - HRP antibody (HRP) (ab21054)

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Overview

Product name

Anti-Histone H3 - HRP antibody (HRP)

Description

Rabbit polyclonal to Histone H3 - HRP (HRP)

Conjugation

HRP

Conjugation notes

This antibody has been conjugated with HRP. The concentration of the original antibody was 0.5 mg/ml, following conjugation the concentration observed will be higher.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster)

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H3.

Read Abcam's proprietary immunogen policy

(Peptide available as ab12149.)

Positive control

This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Yeast S.cerevisiae Whole Cell Lysate Drosophila Embryo Nuclear Extract

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Tags & Cell Markers >> Epitope Tags >> Conjugates
Epigenetics and Nuclear Signaling >> Histones >> H3 >> Unmodified

  • Western blot - Histone H3 - HRP antibody (HRP) (ab21054)Western blot - Histone H3 - HRP antibody (HRP) (ab21054) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab21054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/5000Detects a band...Read more →

    WB: 1/5000Detects a band of approximately 17 kDa (predicted molecular weight: 15.4 kDa).

Target

Relevance

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.

Cellular localization

Nuclear

Alternative names

  • H3 histone antibody
  • H3 histone family member E pseudogene antibody
  • H3.3A antibody
  • H3F3 antibody
  • H3F3A antibody
  • HIST3H3 antibody
  • Histone H3 3 pseudogene antibody
see all

Database links

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Anti-Histone H3 - HRP antibody (HRP) images:

  Western blot - Histone H3 - HRP antibody (HRP) (ab21054)

Western blot - Histone H3 - HRP antibody (HRP) (ab21054)

All lanes : Anti-Histone H3 - HRP antibody (HRP) (ab21054) at 1/5000 dilution

Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179) at 10 µg
Lane 2 : Yeast S.cerevisiae Whole Cell Lysate at 10 µg
Lane 3 : Drosophila Embryo Nuclear Extract at 20 µg


Performed under reducing conditions.

Predicted band size : 15.4 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)

References for Anti-Histone H3 - HRP antibody (HRP) (ab21054)

This product has been referenced in:

  • Wei Wet al. Soluble Frizzled-7 receptor inhibits Wnt signaling and sensitizes hepatocellular carcinoma cells towards doxorubicin. Mol Cancer 10:16 (2011). WB; Human.Read more (PubMed: 21314951) »
  • Hiragami-Hamada Ket al. N-terminal phosphorylation of HP1{alpha} promotes its chromatin binding. Mol Cell Biol 31:1186-200 (2011).Read more (PubMed: 21245376) »

See all 5 publications for this product

Publishing research using ab21054? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"