Histone H3 (K27 modification) Panel (H3, acetyl K27, di methyl K27, tri methyl K27) (ab103964)

Overview

  • Product nameHistone H3 (K27 modification) Panel (H3, acetyl K27, di methyl K27, tri methyl K27)
  • Product overviewab103964 is a Histone H3 (K27 modification) Panel designed for the validation and characterization of the methylation and acetylation state of Histone H3 on K27. Covalent modifications, such as acetylation and methylation, of core histones can contribute to the formation of transcriptionally active and inactive chromatin in response to various signalling pathways and are central modifications for regulating epigenetic transitions in chromatin. Histone H3 methylation on K27 is a repressive modification, and H3 K27 tri-methylation involves recruitment of the Polycomb protein. Histone H3 acetylation on K27 has been shown to identify active enhancers.
  • Components

      Histone H3 (K27 modification) Panel (H3, acetyl K27, di methyl K27, tri methyl K27) images

      • All lanes : Anti-Histone H3 (tri methyl K27) [mAbcam 6002] antibody - ChIP Grade (ab6002) at 1 µg/ml

        Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate
        Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 peptide (ab17163) at 0.5 µg/ml
        Lane 3 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K4) peptide (ab1340) at 0.5 µg/ml
        Lane 4 : Calf Thymus Histone Preparation Nuclear Lysate with Histone H3 (di methyl K4) peptide (ab7768) at 0.5 µg/ml
        Lane 5 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K4) peptide (ab1342) at 0.5 µg/ml
        Lane 6 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 0.5 µg/ml
        Lane 7 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 0.5 µg/ml
        Lane 8 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K9) peptide (ab1773) at 0.5 µg/ml
        Lane 9 : Calf Thymus Histone Preparation Nuclear Lysate with Histone H3 (mono methyl K27) peptide (ab1780) at 0.5 µg/ml
        Lane 10 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K27) peptide (ab1781) at 0.5 µg/ml
        Lane 11 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K27) peptide (ab1782) at 0.5 µg/ml

        Lysates/proteins at 0.5 µg per lane.

        Secondary
        Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Performed under reducing conditions.

        Exposure time : 3 minutes
      • Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab1791 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
      • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab4729 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
      • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab24684 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

      References for Histone H3 (K27 modification) Panel (H3, acetyl K27, di methyl K27, tri methyl K27) (ab103964)

      This product has been referenced in:
      • Erkina TY  et al. Functional interplay between chromatin remodeling complexes RSC, SWI/SNF and ISWI in regulation of yeast heat shock genes. Nucleic Acids Res 38:1441-9 (2010). Read more (PubMed: 20015969) »
      • Verdeguer F  et al. A mitotic transcriptional switch in polycystic kidney disease. Nat Med 16:106-10 (2010). Read more (PubMed: 19966811) »

      See all 2 Publications for this product

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      I am sorry to confirm that the antibody panels are tested in itself and we would not recommend to mix them. On that occasion we can regrettably not give any guarantee for the application to work.

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