Overview
- Product nameHistone H3 (K27 modification) Panel (H3, acetyl K27, di methyl K27, tri methyl K27)
- Product overviewab103964 is a Histone H3 (K27 modification) Panel designed for the validation and characterization of the methylation and acetylation state of Histone H3 on K27. Covalent modifications, such as acetylation and methylation, of core histones can contribute to the formation of transcriptionally active and inactive chromatin in response to various signalling pathways and are central modifications for regulating epigenetic transitions in chromatin. Histone H3 methylation on K27 is a repressive modification, and H3 K27 tri-methylation involves recruitment of the Polycomb protein. Histone H3 acetylation on K27 has been shown to identify active enhancers.
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- Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
- ChIP, ChIP/Chip, CHIPseq, Dot, EM, Flow Cyt, ICC, ICC/IF, IHC - Wmt, IHC-Fr, IHC-P, IP, WB
- Rabbit polyclonal (25µg )
- 1mg/ml
- Reacts with: Ms, Rat, Chk, Dog, Hu, Sc, Xl, At, Ce, Dm, InMtj, S pom, Zfsh, Try c, N cra, Tx gg, Rice, , Chyzon, Frt, S man
- Predicted: Many, Mamm
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- Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729)
- ChIP, ChIP/Chip, CHIPseq, Flow Cyt, ICC/IF, IHC-Fr, IHC-P, PepArr, WB
- Rabbit polyclonal (25µg )
- Reacts with: Ms, Rat, Cow, Hu, At, Ce, Dm, Mk, P fal, Chyzon, Chk
- Predicted: Xl
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- Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab24684)
- ChIP, Dot, Flow Cyt, ICC/IF, IHC - Wmt, IHC-P, IP, WB
- Rabbit polyclonal (25µg )
- 0.4mg/ml
- Reacts with: Ms, Cow, Hu, Ce, Dm, Chyzon, Sc
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- Anti-Histone H3 (tri methyl K27) [mAbcam 6002] antibody - ChIP Grade (ab6002)
- ChIP, ChIP/Chip, Flow Cyt, ICC, ICC/IF, IHC-P, IP, WB
- Mouse monoclonal (25µg )
- 1mg/ml
- Reacts with: Ms, Rat, Cow, Hu, At, Dm, Plnts, Zfsh, RMk
- Predicted: Rb, Chk, Xl, ChHm
Components
Histone H3 (K27 modification) Panel (H3, acetyl K27, di methyl K27, tri methyl K27) images
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Western blot - Histone H3 (K27 modification) Panel (H3, acetyl K27, di methyl K27, tri methyl K27) (ab103964)All lanes : Anti-Histone H3 (tri methyl K27) [mAbcam 6002] antibody - ChIP Grade (ab6002) at 1 µg/ml
Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate
Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 peptide (ab17163) at 0.5 µg/ml
Lane 3 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (mono methyl K4) peptide (ab1340) at 0.5 µg/ml
Lane 4 : Calf Thymus Histone Preparation Nuclear Lysate withHistone H3 (di methyl K4) peptide (ab7768) at 0.5 µg/ml
Lane 5 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (tri methyl K4) peptide (ab1342) at 0.5 µg/ml
Lane 6 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (mono methyl K9) peptide (ab1771) at 0.5 µg/ml
Lane 7 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (di methyl K9) peptide (ab1772) at 0.5 µg/ml
Lane 8 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (tri methyl K9) peptide (ab1773) at 0.5 µg/ml
Lane 9 : Calf Thymus Histone Preparation Nuclear Lysate withHistone H3 (mono methyl K27) peptide (ab1780) at 0.5 µg/ml
Lane 10 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (di methyl K27) peptide (ab1781) at 0.5 µg/ml
Lane 11 : Calf Thymus Histone Preparation Nuclear Lysate withHuman Histone H3 (tri methyl K27) peptide (ab1782) at 0.5 µg/ml
Lysates/proteins at 0.5 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Exposure time : 3 minutes -
ChIPChromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab1791 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region. -
ChIPChromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab4729 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region. -
ChIP - Histone H3 (K27 Modification) Panel (ab103964)Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab24684 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
References for Histone H3 (K27 modification) Panel (H3, acetyl K27, di methyl K27, tri methyl K27) (ab103964)
This product has been referenced in:
- Erkina TY et al. Functional interplay between chromatin remodeling complexes RSC, SWI/SNF and ISWI in regulation of yeast heat shock genes. Nucleic Acids Res 38:1441-9 (2010). Read more (PubMed: 20015969) »
- Verdeguer F et al. A mitotic transcriptional switch in polycystic kidney disease. Nat Med 16:106-10 (2010). Read more (PubMed: 19966811) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"