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RabMAbs (1766-1)

Anti-Histone H3 (acetyl K18) antibody [EP959Y] (ab40888)

Overview

  • Product nameAnti-Histone H3 (acetyl K18) antibody [EP959Y]See all Histone H3 primary antibodies ...
  • Description
    Rabbit monoclonal [EP959Y] to Histone H3 (acetyl K18)
  • SpecificityThis antibody only detects Histone H3 when acetylated on Lysine 18.
  • Tested applicationsICC/IF, WB, IHC-P, ChIP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic acetylated peptide corresponding to residues surronding Lys18 of Histone H3 was used as immunogen.

  • Positive controlC6 cell lysate, human breast carcinoma.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • Clonality Monoclonal
  • Clone numberEP959Y
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab40888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/100 - 1/250.
WB WB: 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P IHC-P: Use at an assay dependent dilution.
ChIP ChIP: Use at an assay dependent concentration. PubMed: 22249179

Target

  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H3 family.
  • Developmental stageExpressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • Post-translational
    modifications
    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • Cellular localizationNucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
      H 3 antibodyH3 3 Like Sequence MH921 antibodyH3 3A antibody
      H3 3A antibodyH3 antibodyH3 Histone antibodyH3 Histone Family Member E Pseudogene antibodyH3.4 antibodyH3/A antibodyH3/g antibodyH31_HUMAN antibodyH3F3 antibodyH3F3 antibodyH3FA antibodyH3FT antibodyH3t antibodyHIST1H3J antibodyHIST3H3 antibodyHIST3H3 antibodyHistone cluster 1, H3a antibodyHistone H3 3 Pseudogene antibodyHistone H3.1 antibodyHistone H3.3 antibodyHistone H3/a antibodyHistone H3/b antibodyHistone H3/c antibodyHistone H3/d antibodyHistone H3/f antibodyHistone H3/h antibodyHistone H3/i antibodyHistone H3/j antibodyHistone H3/k antibodyHistone H3/l antibody
    see all

Anti-Histone H3 (acetyl K18) antibody [EP959Y] images

  • All lanes : Anti-Histone H3 (acetyl K18) antibody [EP959Y] (ab40888) at 1/50000 dilution

    Lane 1 : C6 cell lysate, untreated.
    Lane 2 : C6 cell lysate, treated with TSA.

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 50 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
  • ab40888 diluted 1:100, staining acetylated histone H3 on human breast carcinoma sections.
  • ab40888 (1/500) staining Histone H3 (acetyl K18) in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References for Anti-Histone H3 (acetyl K18) antibody [EP959Y] (ab40888)

This product has been referenced in:
  • Kim JW  et al. New molecular bridge between RelA/p65 and NF-?B target genes via histone acetyltransferase TIP60 cofactor. J Biol Chem 287:7780-91 (2012). ChIP ; Human . Read more (PubMed: 22249179) »
  • Vlaicu SI  et al. Epigenetic modifications induced by RGC-32 in colon cancer. Exp Mol Pathol 88:67-76 (2010). Read more (PubMed: 19883641) »

See all 2 Publications for this product

Product Wall

Displaying 1 - 2 of 2 results for Abreviews and Q&A

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
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Submitted Apr 12 2012

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