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A synthetic acetylated peptide corresponding to residues surrounding Lys 56 of Histone H3
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
This product is available conjugated to Alexa Fluor® 488 see ab170098.
A 40 μl trial size is available to purchase for this antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.
If you have any questions regarding this update, please contact our Scientific Support team.
Our Abpromise guarantee covers the use of ab76307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
|ICC/IF||1/100 - 1/250.|
ab76307 staining Histone H3 (HEK56 Ac) in African Green Monkey kidney epithelial (CV-1 line) cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked with 5% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/500) for 16 hour at 4°C.
CV-1 cells were infected with Simian virus 40 (SV40). 32 hours later the cells were incubated with EdU for 30 minutes before pre-extraction to detect active DNA replication foci and fixed, stained, and visualized by confocal microscopy.
Blue - Hoechst stain, red - anti-T-Ag, magenta - EdU, green - anti-H3K56 Ac. Bar, 5 µm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"