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A synthetic acetylated peptide corresponding to residues surrounding Lys 56 of Histone H3
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Our Abpromise guarantee covers the use of ab76307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/1000 - 1/2000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
|ICC/IF||1/100 - 1/250.|
ab76307 staining Histone H3 (HEK56 Ac) in African Green Monkey kidney epithelial (CV-1 line) cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked with 5% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/500) for 16 hour at 4°C.
CV-1 cells were infected with Simian virus 40 (SV40). 32 hours later the cells were incubated with EdU for 30 minutes before pre-extraction to detect active DNA replication foci and fixed, stained, and visualized by confocal microscopy.
Blue - Hoechst stain, red - anti-T-Ag, magenta - EdU, green - anti-H3K56 Ac. Bar, 5 µm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"