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Anti-Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade (ab12179)

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Overview

Product name

Anti-Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade
See all Histone H3 products (24) ...

Description

Mouse monoclonal [AH3-120] to Histone H3 (acetyl K9) - ChIP Grade

Specificity

Staining of (acetyl K9) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide.

Tested applications

Flow Cyt, ChIP, WB, ELISA, ICC, ICC/IF, IFmore details

Cross reactivity

Reacts with

Mouse, Human, Fruit fly (Drosophila melanogaster), Rice

Predicted to work with

Rat, Chicken, Cow, Xenopus laevis, Caenorhabditis elegans, a wide range of other species

Immunogen

A synthetic, acetylated histone H3 peptide (amino acids 7-20, acetyl K9) corresponding to the N-terminus of human histone H3, conjugated to KLH.

General notes

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

AH3-120

Myeloma

NS1

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Histones >> H3 >> Acetylated

  • Immunocytochemistry - Histone H3 (acetyl K9) antibody [AH3-120] (ab12179)Immunocytochemistry - Histone H3 (acetyl K9) antibody [AH3-120] (ab12179) image (enlarge)

  • ChIP - Histone H3 (acetyl K9) antibody [AH3-120] (ab12179)ChIP - Histone H3 (acetyl K9) antibody [AH3-120] (ab12179) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade (ab12179)Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade (ab12179) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ChIP: Use 5-10µg for 25µg of chromatin
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution (PMID 19584087).
ICC: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent concentration.
IF: 1/1000.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 17 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Sequence similarities

Belongs to the histone H3 family.

Developmental stage

Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

Post-translational
modifications

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.

Cellular localization

Nucleus. Chromosome.

Target information above from: UniProt accessionP68431 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • H3FA antibody
  • H 3 antibody
  • H3 3 Like Sequence MH921 antibody
  • H3 3A antibody
  • H3 3A antibody
  • H3 antibody
  • H3 Histone antibody
  • H3 Histone Family Member E Pseudogene antibody
  • H3.4 antibody
  • H3/A antibody
  • H3/g antibody
  • H31_HUMAN antibody
  • H3F3 antibody
  • H3F3 antibody
  • H3FT antibody
  • H3t antibody
  • HIST1H3J antibody
  • HIST3H3 antibody
  • HIST3H3 antibody
  • Histone cluster 1, H3a antibody
  • Histone H3 3 Pseudogene antibody
  • Histone H3.1 antibody
  • Histone H3.3 antibody
  • Histone H3/a antibody
  • Histone H3/b antibody
  • Histone H3/c antibody
  • Histone H3/d antibody
  • Histone H3/f antibody
  • Histone H3/h antibody
  • Histone H3/i antibody
  • Histone H3/j antibody
  • Histone H3/k antibody
  • Histone H3/l antibody
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Database links

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Anti-Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade images:

  Immunocytochemistry - Histone H3 (acetyl K9) antibody [AH3-120] (ab12179)

Immunocytochemistry - Histone H3 (acetyl K9) antibody [AH3-120] (ab12179)

SK-N-SH cells fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab12179 (1/1000 dilution). ab12179 staining is localized to the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.

Darin McDonald, Cross Cancer Institute

  ChIP - Histone H3 (acetyl K9) antibody [AH3-120] (ab12179)

ChIP - Histone H3 (acetyl K9) antibody [AH3-120] (ab12179)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 5µg of  ab12179 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
    

  Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade (ab12179)

Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade (ab12179)

ab12179 at a 1/1000 dilution staining Stylonychia lemnae (single cell organism, transcriptionally active macronucleus) by ICC/IF. The cells were paraformaldehyde fixed and incubated with the antibody for 12 hours. An Alexa Fluor ® 488 conjugated goat anti-mouse IgG antibody was used as the secondary.

In the image Histone H3 (acetyl K9) staining is red and is found in macronuclei only. Micronuclei remain unstained and are shown in blue (nucleic acid counterstain). Alpha tubulin is also stained (green).

This image is courtesy of an Abreview submitted by Dr Jan Postberg

See Abreview

References for Anti-Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade (ab12179)

This product has been referenced in:

  • Zhong Let al. The Histone Deacetylase Sirt6 Regulates Glucose Homeostasis via Hif1alpha. Cell 140:280-293 (2010). ChIP; Mouse.Read more (PubMed: 20141841) »
  • Marshall Het al. Deficiency of the dual ubiquitin/SUMO ligase Topors results in genetic instability and an increased rate of malignancy in mice. BMC Mol Biol 11:31 (2010). ICC/IF; Mouse.Read more (PubMed: 20429939) »

See all 6 publications for this product

Publishing research using ab12179? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"