Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)

I am interested to buy Anti-Histone H3 (acetyl K9) antibody to use it in my ChIP experiment. but the thing is you have 3 H3K9 recommended for ChIP. 1- ab4441 2- ab10812 3- ab113674 Which one do you recommend is the best one for ChIP?

ANSWER:

Thank you for contacting us.

Antibody ab113674 is the newest antibody of the three and is therefore less thoroughly characterized. Antibodies ab4441 and ab10812 have both been published many times and have received positive customer reviews for ChIP, among other applications. Ab4441 is the more popular of the two, most likely because it has many more references.

Please do not hesitate to contact us if you need any more advice or information.

What volume we will get for ab191 and ab4441.

ANSWER:

Thank you for contacting us.

The volume of product will depend on the concentration of product, which is currently 1mg/ml. At 1 mg/ml the volume of ab1791 will be 100ul and for ab4441is 50ul.

Please also add ab123069 in your order which we are giving away free of charge with all primary orders.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Ab4441cross-reacts with histone H3 acetyl K18, 20, and 27 in a peptide array.

ANSWER:

Your colleague called today to let us know about an issue with the quality of ab4441 on order ***, and I have asked our accounting department to issue credit note *** for you. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further assistance.

Anyway, is it possible for you to supply >1mg of antibody per lot? Furthermore, what is the maximum you can supply per lot?  In my eyes, I am looking for as much QC data as possible (western blot, chip, cross-reactivity dot blot, protein quantification, etc); I am not worried about your internal control QC but just what is relevant for a functional specific antibody. I would still like to receive the QC data on the lot no. that are now out of stock, if it is possible.  

ANSWER:

Thank you for your reply.

I have emailed the lab to send me all the QC data for the antibodies and lots you requested, and I will pass them on to you when I hear back from the lab.

Yes, 1 mg would be possible, but it depends on the lot and antibody. For some of the lots you inquired about we might have more than 1 mg in stock, for others it would be less than a mg. This also changes daily as other customer purchase vials. But in general it would be possible, but it is lot and antibody-dependent. For more detailed information of what is available, please contact my colleague Andrea at sales@abcam.com as she is specialized in bulk orders and arranging them for our customers.

Thank you for your patience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Hi Abcam, I can't find information about whether your antibodies (specifically Ab4441) are compatible with magnetic G beads - I understand from the Antibody information pages that your antibodies are compatible with sepharose beads, but I'm not sure if this includes magnetic sepharose beads too? I have magnetic beads XXX and would like to use them with your antibodies in a ChIP seq experiment. Will this be compatible? I'm new to this field, so thanks in advance for your help!

ANSWER:

Thank you very much for your inquiry. Indeed, our antibodies (as all normally purified antibodies) are in principle very well compatible with magnetic beads as well.  Indeed, as long as the beads are coated to protein G and the antibody will therefore be bound by protein G, there is no problem and it will work well also if the beads are magnetic. The ab4441 is a rabbit polyclonal antibody and will therefore be bound by protein G. Please find also a list of the types of antibodies bound by protein G on our IP protocol: http://www.abcam.com/index.html?pageconfig=resource&rid=11385 I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.