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ChIP - Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
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Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
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Western blot - Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
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Product Name
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Histone H3 (acetyl K9) antibody - ChIP Grade
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See all Histone H3 (acetylated) antibodies (20)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Histone H3 (acetyl K9) - ChIP Grade
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Immunogen
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Synthetic peptide: ARTKQTARAcKSTG-C conjugated to KLH, corresponding to amino acids 1-12 of Human Histone H3.
BLAST 'ARTKQTARAcKSTG-C' with  or 
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Reacts with
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Hu, Ms, Ce, Dm, S pom, Tet, Tx gg, Xl
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Predicted to react
(species key)
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Sc
(Due to sequence homology)
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Specificity
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In Dot blot detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3.
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Tested applications
(see key)
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CHIPseq, ChIP, Dot, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use 2µg for 106 cells.
CHIPseq: Use at an assay dependent dilution.
Dot: Use at a concentration of 0.5 µg/ml.
ICC/IF: 1/100 - 1/200.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 17 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: acid extracted proteins from HeLa cells
ICC:polytene chromosomes of Drosophila melanogaster
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> H3 >> Acetylated
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Relevance
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Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Modification results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. Histone deacetylation by histone deacetylases (HDACs) is associated with transcription repression reversing the chromatin remodeling process. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide - made up to 30% glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 (acetyl K9) antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 (acetyl K9) antibody - ChIP Grade images, references, products related to ab4441 and other tools.
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Histone H3 (acetyl K9) antibody - ChIP Grade images:
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 ChIP - Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4441 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
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Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
Fruit fly (Drosophila melanogaster) Cell (polytene chromosomes) were fixed in formaldehyde, blocked in 1% BSA for 30 minutes and incubated with ab4441 (1/100) for 12 hours. This image was submitted as part of a review by review by Anita Ciurciu.
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Western blot - Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
Ab4441, Histone H3, acetylated (Lys9) Pab Rabbit x Human WB: Untreated (lane 1) and treated with sodium butyrate (lane 2) HeLa cell acid-extract was probed with H5110-14A at 0.5ug/ml.
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References for Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
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This product has been used in: (two most recent references)
Verdeguer F et al. A mitotic transcriptional switch in polycystic kidney disease. Nat Med 16:106-10 (2010). ChIP; Mouse. PubMed: 19966811
Sun J & Li R Human negative elongation factor activates transcription and regulates alternative transcription initiation. J Biol Chem 285:6443-52 (2010). ChIP; Human. PubMed: 20028984
See all 51 publication references for this product.
If you publish research using ab4441 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Histone H3
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