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Synthetic peptide: ARTKQTARAcKSTG-C conjugated to KLH, corresponding to amino acids 1-12 of Human Histone H3.
Our Abpromise guarantee covers the use of ab4441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200.|
|CHIPseq||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent concentration.|
|Dot Blot||Use a concentration of 0.5 µg/ml. Detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3.|
|WB||1/500. Detects a band of approximately 17 kDa.|
|ChIP||Use 2µg for 106 cells.|
|IHC-P||Use at an assay dependent concentration.|
Fruit fly (Drosophila melanogaster) Cell (polytene chromosomes) were fixed in formaldehyde, blocked in 1% BSA for 30 minutes and incubated with ab4441 (1/100) for 12 hours.
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4441 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
ab4441, Histone H3, acetylated (Lys9) Pab Rabbit x Human
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