Products:Epigenetics and Nuclear Signaling >> Histones >> H3 >> Unmodified
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ab46666 |
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ab12079 has been referenced in 3 publications.
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Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab12079 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Anti-Histone H3 antibody - ChIP Grade (ab12079) at 0.5 µg/ml + HeLa Cell Histone prep at 5 µg
Secondary
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
All lanes : Anti-Histone H3 antibody - ChIP Grade (ab12079) at 1 µg/ml
Lane 1 : Chicken S1:29 Cell lysate at 10 µg
Lane 2 : CHO Cell lysate at 10 µg
Lane 3 : Dog MDCK Cell lysate at 10 µg
Lane 4 : Mouse 3T3 Whole Cell Lysate
Lane 5 : Xenopus embryo Cell lysate at 10 µg
Secondary
Alexa Fluor 680 Rabbit anti-Goat IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
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