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Western blot - Histone H3 antibody - ChIP Grade (ab1791)
Western blot - Histone H3 antibody - ChIP Grade (ab1791)
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ChIP - Histone H3 antibody - ChIP Grade (ab1791)
ChIP - Histone H3 antibody - ChIP Grade (ab1791)
(enlarge)


ChIP - Histone H3 antibody - ChIP Grade (ab1791)
ChIP - Histone H3 antibody - ChIP Grade (ab1791)
(enlarge)


Product Name 

Histone H3 antibody - ChIP Grade

 

See all Histone H3 - unmodified antibodies (20)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Histone H3 - ChIP Grade

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H3.

(Peptide available as ab12149.)

Reacts with 

Hu, Ms, Rat, At, Ce, Chk, Dog, Dm, InMtj, N cra, Sc, S pom, Tx gg, Try c, Xl

Predicted to react 
(species key)

Many, Mamm
(Due to sequence homology)

Tested applications 
(see key)

CHIPseq, ChIP, ChIP/Chip, Dot, Flow Cyt, ICC/IF, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ChIP

23 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• Flow Cyt

1 Abreview

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• ICC

1 Abreview

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• ICC/IF

3 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)

• IHC-P

3 Abreviews

- average rating: 5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)5 stars (out of 5)

• WB

32 Abreviews

- average rating: 4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)4 stars (out of 5)


WB
(read Abreview)


ChIP
(read Abreview)


WB
(read Abreview)


ChIP
(read Abreview)


Application notes 
(see key)

Recommended dilutions
CHIPseq: Use at an assay dependent dilution.
ChIP: Use 2µg for 106 cells.
ChIP on chip: Use at an assay dependent dilution.
Dot: 1/10,000.
Flow Cyt: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IF: 1/500.
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution.
WB: 1/1000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa). Can be blocked with Histone H3 peptide (ab12149).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> H3 >> Unmodified

Relevance 

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.

 

Alternative names Histone H3 antibody - ChIP Grade (ab1791)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

176359    (Caenorhabditis elegans)
3020    (Human)
3021    (Human)
15079    (Mouse)
15080    (Mouse)

GeneCard

    

GC01P224316    (Human)
GC15U900530    (Human)
GC14U900594    (Human)

Omim

    

601128    (Human)

SwissProt

    

P59226    (Arabidopsis thaliana)
P08898    (Caenorhabditis elegans)
Q10453    (Caenorhabditis elegans)
P06351    (Fruit fly (Drosophila melanogaster))
P02299    (Fruit fly (Drosophila melanogaster))
Q93081    (Human)
Q16695    (Human)
P06351    (Human)
P16106    (Human)
P16106    (Human)
P84243    (Human)
P16105    (Mouse)
P07041    (Neurospora crassa )
P06351    (Rat)
P02303    (Saccharomyces cerevisiae)
P16105    (Xenopus laevis)

Unigene

    

533624    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.50 - 1.00 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance!


See below for Histone H3 antibody - ChIP Grade images, references, products related to ab1791 and other tools.

 

Histone H3 antibody - ChIP Grade images:

Product image  Western blot - Histone H3 antibody - ChIP Grade (ab1791)

Western blot - Histone H3 antibody - ChIP Grade (ab1791)

All lanes : Histone H3 antibody - ChIP Grade (ab1791) at 1/1000 dilution

Lane 1 : A431 Whole Cell Lysate
Lane 2 : Jurkat Whole Cell Lysate
Lane 3 : 293 Whole Cell Lysate
Lane 4 : A431 Whole Cell Lysate with Histone H3 peptide (ab12149) at 1 µg/ml
Lane 5 : Jurkat Whole Cell Lysate with Histone H3 peptide (ab12149) at 1 µg/ml
Lane 6 : 293 Whole Cell Lysate with Histone H3 peptide (ab12149) at 1 µg/ml

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)


Exposure time : 10 seconds

 

Product image  ChIP - Histone H3 antibody - ChIP Grade (ab1791)

ChIP - Histone H3 antibody - ChIP Grade (ab1791)

Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab1791 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

 

Product image  ChIP - Histone H3 antibody - ChIP Grade (ab1791)

ChIP - Histone H3 antibody - ChIP Grade (ab1791)

Hela cells cultured on coverslips were fixed with 4% paraformaldehyde and then stained with ab1791 (green) at a working dilution of 1/500. The DNA stained with DAPI is shown in red. (100x magnification).

Kirk McManus, University of British Columbia

 

Product image  ChIP - Histone H3 antibody - ChIP Grade (ab1791)

ChIP - Histone H3 antibody - ChIP Grade (ab1791)

Chromatin from Xenopus laevis oocytes was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10 min. The ChIP was performed with 25 mg of chromatin, 3 mg of ab7834 (anti-H3, light blue) and 3 µg of ab1791 (anti-H3, dark blue), and 20 ml of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

 

Product image  Flow Cytometry - Histone H3 antibody - ChIP Grade (ab1791)

Flow Cytometry - Histone H3 antibody - ChIP Grade (ab1791)

 ab1791 staining mouse embryonic stem cells by flow cytometry (gated on all living cells). The cell colonies were trypsinized and incubated with the antibody 1ug/1.5 x 105cells in a permeabilization buffer. A PE conjugated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Prof Albrecht Müller

See Abreview

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 antibody - ChIP Grade (ab1791)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 antibody - ChIP Grade (ab1791)

ab1791 staining rat testes tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking with 5% serum for 30 minutes at 22°C.  The primary antibody was diluted 1/400 and incubated with the sample for 30 minutes at 22°C.  A HRP-conjugated goat anti-rabbit antibody diluted 1/400 was used as the secondary.

This image is courtesy of an anonymous Abreview

See Abreview

 

Product image  Western blot - Histone H3 antibody - ChIP Grade (ab1791)

Western blot - Histone H3 antibody - ChIP Grade (ab1791)

All lanes : Histone H3 antibody - ChIP Grade (ab1791) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 3 : Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr)
Lane 4 : S.cerevisiae (Y190) Whole Cell Lysate
Lane 5 : S.pombe Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)


ab1791 is tested in western blot on a range of species.  We recommend loading higher amounts of protein (20-30ug) to increase the signal in yeast lysates

 

Product image  Immunocytochemistry/ Immunofluorescence - Histone H3 antibody - ChIP Grade (ab1791)

Immunocytochemistry/ Immunofluorescence - Histone H3 antibody - ChIP Grade (ab1791)

ICC/IF image of ab1791 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1791, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 5% PFA fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa and Hek293 cells at 1µg/ml.

 

Product image  Western blot - Histone H3 antibody - ChIP Grade (ab1791)

Western blot - Histone H3 antibody - ChIP Grade (ab1791)



Predicted band size : 15 kDa

John E. Mueller and J. Ruth German (Mary Bryk lab)

 

References for Histone H3 antibody - ChIP Grade (ab1791)

Specific publication references   This product has been used in: (two most recent references)

Mehedint MG et al. Choline deficiency alters global histone methylation and epigenetic marking at the Re1 site of the calbindin 1 gene. FASEB J 24:184-95 (2010). WB; Mouse. PubMed: 19752176

Kumar SV & Wigge PA H2A.Z-Containing Nucleosomes Mediate the Thermosensory Response in Arabidopsis. Cell 140:136-147 (2010). ChIP; Arabidopsis thaliana. PubMed: 20079334


See all 247 publication references for this product.


If you publish research using ab1791 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Histone H3

 

Histone H3 antibody - ChIP Grade - more information

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Histone H3 antibody - ChIP Grade - related products:

Products for ChIP

ChIP Related Products

Products for Immunocytochemistry

Subcellular Markers

Products for Immunofluorescence

Subcellular Markers

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

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Immunizing Peptide (Blocking) for Histone H3 antibody - ChIP Grade

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Histone H3 peptide

 

Immunizing Peptide for Histone H3 antibody - ChIP Grade

 

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Other Histone H3 Antibodies

See all Histone H3 - unmodified antibodies (20)...

 

Histone H3 antibody - ChIP Grade - other tools:

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