Anti-Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)

Dear technical support:

This customer raised an inquiry about “Total histone H3 antibody”, this customer wants to used this antibody conduct the IFassay with human sample, and she also indicated the host of this antibody can’t be rabbit; therefore, could you please offer any suitable selection to this customer?

Thanks for your kindly assistance.

ANSWER:

Thank you very much for your interest in our Histone H3 antibodies.

I am happy to let you know that we have non-rabbit anti-human Histone H3 antibodies available, which also qualify for our testing discount programme (either because of their potential suitability for IF or because we do not have an image yet).

These are:

ab10799: mouse monoclonal, suitable for IHC, so might work for ICC/IF

http://www.abcam.com/index.html?datasheet=10799 (or use the following: http://www.abcam.com/index.html?datasheet=10799).

ab128012: sheep polyclonal, suitable for ICC/IF, no image yet

http://www.abcam.com/index.html?datasheet=128012 (or use the following: http://www.abcam.com/index.html?datasheet=128012).

Also, I could recommend our favourite rabbit polyclonal ab1791. I assume the customer is performing a costaining with another rabbit antibody and thus wants to avoid a rabbit anti-Histone H3 antibody. However, I am pleased to let you know that with our Easy Link kits this problem can be solved: You can easily label any primary antibody with a conjugate of your choice and eliminate the need for secondary antibodies. Please find more information under http://www.abcam.com/Easylink.

I am sure you are aware of our testing discount scheme which applies to the above mentioned ab10799 and ab128012:

I can offer a discount off a future purchase if you buy the antibody now, test it and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and which antibody you would like to test. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

3. Test it.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thanks for your help with that order. I have another question for an additional project we are initiating requiring lots of different antibodies. This is a clinical diagnostics projects, and all antibodies we will use for that need to be CE certified for diagnostic purposes. Since I did not find any information on this on your website, I wanted to ask you if you have any CE certified antibodies directed against these (human) proteins:

ANSWER:

Thank you for contacting Abcam.

All of our products are for research purposes only and are not certified for clinical research applications.

I am sorry that I could not be more helpful in this matter.

will test ab10799 in ELISA
will test ab80256 in ELISA

ANSWER:

Thank you for your phone call. As we discussed I am sending you the discount codes for the 2 antibodies you would like to try in ELISA.
DISCOUNT CODE: xxx for ab10799
DISCOUNT CODE: xxx for ab80256
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab10799 and ab80256 in ELISA. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ELISAand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: http://www.abcam.com/collaborationdiscount.

I ordered 2 vials of this antibody and was expecting to receive 200 ug, but I only received 36 ug in each vial, for a total of 72 ug.

ANSWER:

I am sorry that you did not receive the proper amount of antibody that you ordered and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of 3 vials for an additional 174 ug. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

I'll like to know if this antibody recognized all forms of histone H3 sush as the variant H3.3? Is it possible to get the amino sequence of the epitope recognized by this antibody or the amino acid sequence of the immunizing peptide?

ANSWER:

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

However, the homology of the immunogen and H3 variants: H3.1, H3.2, H3.3 is 100%.

Should you have any further questions, please do not hesitate to contact us.