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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)](/ps/datasheet/images/ab10799-ap402864.jpg)
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Western blot - Histone H3 antibody [mAbcam 10799] (ab10799)
![Western blot - Histone H3 antibody [mAbcam 10799] (ab10799)](/ps/datasheet/images/ab10799_1.jpg)
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ChIP - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
![ChIP - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)](/ps/datasheet/images/ab10799_3.jpg)
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Product Name
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Histone H3 antibody [mAbcam 10799] - ChIP Grade
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See all Histone H3 - unmodified antibodies (20)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [mAbcam 10799] to Histone H3 - ChIP Grade
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Immunogen
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Synthetic peptide derived from within residues 100 to the C-terminus of Human Histone H3. Generated with the same immunogen as our successful rabbit polyclonal ab1791.
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Reacts with
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Hu, Ms, Dm, Mk
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Predicted to react
(species key)
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Many, Mamm
(Due to sequence homology)
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Tested applications
(see key)
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ChIP, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use 2-4µg for 25µg of chromatin
ICC/IF: Use at a concentration of 5-10 µg/ml.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa). Can be blocked with Histone H3 peptide (ab12149).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Histones >> H3 >> Unmodified
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Relevance
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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mAbcam 10799
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Myeloma
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Sp2/0-Ag14
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Isotype
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IgG3
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 antibody [mAbcam 10799] - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 antibody [mAbcam 10799] - ChIP Grade images, references, products related to ab10799 and other tools.
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Histone H3 antibody [mAbcam 10799] - ChIP Grade images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
IHC image of Histone H3 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10799, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Histone H3 antibody [mAbcam 10799] (ab10799)
Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799) at 1 µg/ml + Jurkat cell lysate at 20 µg
Secondary
Goat polyclonal to Mouse IgG - H&L (HRP) (ab6789) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 15 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
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ChIP - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab10799 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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Immunocytochemistry/ Immunofluorescence - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
![Immunocytochemistry/ Immunofluorescence - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)](/ps/datasheet/images/ab10799-IF-Im2.jpg)
ICC/IF image of ab10799 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab10799, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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References for Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)
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This product has been used in: (two most recent references)
Peng H et al. Transcriptional coactivator HCF-1 couples the histone chaperone Asf1b to HSV-1 DNA replication components. Proc Natl Acad Sci U S A 107:2461-6 (2010). ICC/IF; Monkey. PubMed: 20133788
Lee N et al. The H3K4 demethylase lid associates with and inhibits histone deacetylase Rpd3. Mol Cell Biol 29:1401-10 (2009). WB; Fruit fly (Drosophila melanogaster). PubMed: 19114561
See all 3 publication references for this product.
If you publish research using ab10799 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Histone H3
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Histone H3 antibody [mAbcam 10799] - ChIP Grade - more information
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Histone H3 antibody [mAbcam 10799] - ChIP Grade - related products:
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Histone H3 antibody [mAbcam 10799] - ChIP Grade - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)](/index.html?datasheet=10799#ab10799-ap402864.jpg)
![Western blot - Histone H3 antibody [mAbcam 10799] (ab10799)](/index.html?datasheet=10799#ab10799_1.jpg)
![ChIP - Histone H3 antibody [mAbcam 10799] - ChIP Grade (ab10799)](/index.html?datasheet=10799#ab10799_3.jpg)
