Histone H3 antibody [mAbcam 24834] (ab24834)

Product Name 

Histone H3 antibody [mAbcam 24834]

Product type 

Primary antibodies

Description 

Mouse monoclonal [mAbcam 24834] to Histone H3

Immunogen 

Synthetic peptide derived from within residues 100 to the C-terminus of Human Histone H3. Generated with the same immunogen as our successful rabbit polyclonal ab1791.

(Peptide available as ab12149.)

Reacts with 

Hu

Predicted to react 
(species key)

Many, Mamm
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.5-1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa). Can be blocked with Histone H3 peptide - 124-135 (ab12149).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Histones >> H3 >> Unmodified

Relevance 

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by histone modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mAbcam 24834

Myeloma 

Sp2/0-Ag14

Isotype 

IgG3

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.5

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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