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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Histones >> H3 >> Citrullated
Anti-Histone H3 (citrulline 2 + 8 + 17) antibody
See all Histone H3 products (4) ...
Rabbit polyclonal to Histone H3 (citrulline 2 + 8 + 17)
This antibody reacts specifically with human Histone H3 Citrulline at either position: 2 and/or 8 and/or 17.
WB, IHC-P, ICC/IFmore details
Reacts with
Human
Synthetic peptide derived from the N terminal region of Human Histone H3, containing Citrulline at position 2 + 8 + 17.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: Whole serum
Whole antiserum
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Histones >> H3 >> Citrullated
Our Abpromise guarantee covers the use of ab77164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200 - 1/1000.Predicted molecular weight: 15 kDa.
IHC-P: 1/100 - 1/500.
ICC/IF: Use at an assay dependent dilution. (PubMed: 20818377)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Methylation of arginine residues within histone H3 has been linked to active transcription. However, the mechanism of demethylation of histones has remained elusive. Recently a process termed deimination has been described that converts histone arginine to citrulline and antagonizes arginine methylation (Cuthbert et al., 2004).
Nuclear
Western blot - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

All lanes : Anti-Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164) at 1/10000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Human histone protein
Predicted band size : 15 kDa
Observed band size : 15 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

ab80256 at 1/1000 dilution staining Histone H3 (Citrulline 2 + 8 + 17) in HL60 cells by Immunocytochemistry/ Immunofluorescence. HL60 cells treated with DMSO and calcimycin. The primary antibody show green staining in image and nucleus stained blue with Hoechst.
This product has been referenced in:
See all 2 publications for this product
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All lanes : Anti-Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164) at 1/10000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Human histone protein
Predicted band size : 15 kDa
Observed band size : 15 kDa

ab80256 at 1/1000 dilution staining Histone H3 (Citrulline 2 + 8 + 17) in HL60 cells by Immunocytochemistry/ Immunofluorescence. HL60 cells treated with DMSO and calcimycin. The primary antibody show green staining in image and nucleus stained blue with Hoechst.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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