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Anti-Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

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Overview

Product name

Anti-Histone H3 (citrulline 2 + 8 + 17) antibody
See all Histone H3 products (4) ...

Description

Rabbit polyclonal to Histone H3 (citrulline 2 + 8 + 17)

Specificity

This antibody reacts specifically with human Histone H3 Citrulline at either position: 2 and/or 8 and/or 17.

Tested applications

WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide derived from the N terminal region of Human Histone H3, containing Citrulline at position 2 + 8 + 17.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Histones >> H3 >> Citrullated

  • Western blot - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)Western blot - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab77164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/200 - 1/1000.Predi...Read more →

    WB: 1/200 - 1/1000.Predicted molecular weight: 15 kDa.

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/500.

    IHC-P: 1/100 - 1/500.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (PubMed: 20818377)

Target

Relevance

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Methylation of arginine residues within histone H3 has been linked to active transcription. However, the mechanism of demethylation of histones has remained elusive. Recently a process termed deimination has been described that converts histone arginine to citrulline and antagonizes arginine methylation (Cuthbert et al., 2004).

Cellular localization

Nuclear

Alternative names

  • Cit Histone H3 antibody
  • Citrullated antibody
  • citrullin antibody
  • Citrullinated Histone H3 antibody
  • citrulline antibody
  • H3 3 like sequence MH921 antibody
  • H3 3A antibody
  • H3 histone antibody
  • H3 histone family member E pseudogene antibody
  • H3F3 antibody
  • HIST3H3 antibody
  • Histone H3 3 pseudogene antibody
  • Histone H3 Citrullated antibody
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Database links

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Anti-Histone H3 (citrulline 2 + 8 + 17) antibody images:

  Western blot - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

Western blot - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

All lanes : Anti-Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164) at 1/10000 dilution

Lane 1 : Molecular weight marker
Lane 2 : Human histone protein


Predicted band size : 15 kDa
Observed band size : 15 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

ab80256 at 1/1000 dilution staining Histone H3 (Citrulline 2 + 8 + 17) in HL60 cells by Immunocytochemistry/ Immunofluorescence. HL60 cells treated with DMSO and calcimycin. The primary antibody show green staining in image and nucleus stained blue with Hoechst.

References for Anti-Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

This product has been referenced in:

  • Marcos Vet al. CXCR2 mediates NADPH oxidase-independent neutrophil extracellular trap formation in cystic fibrosis airway inflammation. Nat Med 16:1018-23 (2010). ICC/IF; Human.Read more (PubMed: 20818377) »
  • Cherrington BDet al. Potential role for peptidylarginine deiminase 2 (PAD2) in citrullination of canine mammary epithelial cell histones. PLoS One 5:e11768 (2010). WB; Dog.Read more (PubMed: 20668670) »

See all 2 publications for this product

Publishing research using ab77164? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"