Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

Product Name 

Histone H3 (citrulline 2 + 8 + 17) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Histone H3 (citrulline 2 + 8 + 17)

Immunogen 

Synthetic peptide derived from the N terminal region of Human Histone H3, containing Citrulline at position 2 + 8 + 17.

Reacts with 
(species key)

Hu

Specificity 

This antibody reacts specifically with human Histone H3 Citrulline at either position 2 and or 8 and or 17. This antibody does not cross react with mono or bis citrulination.

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/100 - 1/500.
WB: 1/200 - 1/1000. Predicted molecular weight: 15 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Histones >> H3 >> Citrullated

Relevance 

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Methylation of arginine residues within histone H3 has been linked to active transcription. However, the mechanism of demethylation of histones has remained elusive. Recently a process termed deimination has been described that converts histone arginine to citrulline and antagonizes arginine methylation (Cuthbert et al., 2004).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

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References for Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

  This product has been used in: (two most recent references)

Raijmakers R et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol 367:1118-29 (2007). PubMed: 17303166

Mastronardi FG et al. Increased citrullination of histone H3 in multiple sclerosis brain and animal models of demyelination: a role for tumor necrosis factor-induced peptidylarginine deiminase 4 translocation. J Neurosci 26:11387-96 (2006). PubMed: 17079667
If you publish research using ab77164 please let us know so that we can cite the reference on this datasheet.

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