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Western blot - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)

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Product Name
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Histone H3 (citrulline 2 + 8 + 17) antibody
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See all Histone H3 (Citrullated) antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Histone H3 (citrulline 2 + 8 + 17)
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Immunogen
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Synthetic peptide derived from the N terminal region of Human Histone H3, containing Citrulline at position 2 + 8 + 17.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts specifically with human Histone H3 Citrulline at either position 2 and or 8 and or 17. This antibody does not cross react with mono or bis citrulination.
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-P: 1/100 - 1/500. WB: 1/200 - 1/1000. Predicted molecular weight: 15 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Histones >> H3 >> Citrullated
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Relevance
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Methylation of arginine residues within histone H3 has been linked to active transcription. However, the mechanism of demethylation of histones has remained elusive. Recently a process termed deimination has been described that converts histone arginine to citrulline and antagonizes arginine methylation (Cuthbert et al., 2004).
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 (citrulline 2 + 8 + 17) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 (citrulline 2 + 8 + 17) antibody images, references, products related to ab77164 and other tools.
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Histone H3 (citrulline 2 + 8 + 17) antibody images:
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Western blot - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)
Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164) at 1/10000 dilution
Predicted band size : 15 kDa Observed band size : 15 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)
HL60 cells treated with DMSO and Calcimycin.
Green: ab77164 (1:1000).
Blue: Hoechst.
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References for Histone H3 (citrulline 2 + 8 + 17) antibody (ab77164)
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This product has been used in: (two most recent references)
Raijmakers R et al. Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro. J Mol Biol 367:1118-29 (2007). PubMed: 17303166
Mastronardi FG et al. Increased citrullination of histone H3 in multiple sclerosis brain and animal models of demyelination: a role for tumor necrosis factor-induced peptidylarginine deiminase 4 translocation. J Neurosci 26:11387-96 (2006). PubMed: 17079667
If you publish research using ab77164 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Histone H3
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Histone H3 (citrulline 2 + 8 + 17) antibody - more information
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Histone H3 (citrulline 2 + 8 + 17) antibody - related products:
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Histone H3 (citrulline 2 + 8 + 17) antibody - other tools:
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Contact Abcam with a Technical Enquiry about Histone H3 (citrulline 2 + 8 + 17) antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"