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Product Name
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Histone H3 (citrulline R26) antibody
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See all Histone H3 (Citrullated) antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Histone H3 (citrulline R26)
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Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3.
(Peptide available as ab20631.)
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Abreviews
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Application notes
(see key)
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Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide (ab20631) . It gave a negative result in ELISA against the non-modified equivalent peptide (ab2623). This indicates that it is specific for the modified peptide.
See figure below.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Histones >> H3 >> Citrullated
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Relevance
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Methylation of arginine residues within histone H3 has been linked to active transcription. However, the mechanism of demethylation of histones has remained elusive. Recently a process termed deimination has been described that converts histone arginine to citrulline and antagonizes arginine methylation (Cuthbert et al., 2004).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Histone H3 (citrulline R26) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Histone H3 (citrulline R26) antibody images, references, products related to ab19847 and other tools.
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Histone H3 (citrulline R26) antibody images:
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This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'
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 ELISA - Histone H3 (citrulline R26) antibody (ab19847)
ab19847 gave a positive result in ELISA against the immunising peptide (ab20631) and a negative result using the non-modified equivalent peptide (ab2623). This indicates that ab19847 is specifically recognising Histone H3 citrulline R26.
Green and Orange lines: ELISA against immunising peptide (ab20631)
Red and Mauve lines: ELISA against non-modified peptide (ab2623)
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Search PubMed (MEDLINE) for references to Histone H3
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Histone H3 (citrulline R26) antibody - more information
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Customer reviews (feedback) regarding Histone H3 (citrulline R26) antibody |
Customer FAQs regarding Histone H3 (citrulline R26) antibody |
Protocols for Histone H3 (citrulline R26) antibody |
Price and availability of products related to Histone H3 (citrulline R26) antibody |
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Histone H3 (citrulline R26) antibody - related products:
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Histone H3 (citrulline R26) antibody - other tools:
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Contact Abcam with a Technical Enquiry about Histone H3 (citrulline R26) antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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