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Products:Epigenetics and Nuclear Signaling >> Histones >> H3 >> Citrullated
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-Histone H3 (citrulline R26) antibody
See all Histone H3 products (4) ...
Description
Rabbit polyclonal to Histone H3 (citrulline R26)
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3.
(Peptide available as ab20631.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Histones >> H3 >> Citrullated
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ELISA - Histone H3 (citrulline R26) antibody (ab19847)
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Applications
Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab20631) . It gave a negative result in ELISA against the non-modified equivalent peptide (ab2623). This indicates that it is specific for the modified peptide.
See figure below.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Methylation of arginine residues within histone H3 has been linked to active transcription. However, the mechanism of demethylation of histones has remained elusive. Recently a process termed deimination has been described that converts histone arginine to citrulline and antagonizes arginine methylation (Cuthbert et al., 2004).
Cellular localization
Nuclear
Alternative names
- Cit Histone H3 antibody
- Citrullated antibody
- citrullin antibody
see all
Database links
- Entrez Gene: 10338 Human
- Entrez Gene: 3020 Human
- Entrez Gene: 3021 Human
- Omim: 601128 Human
- SwissProt: P06351 Human
- SwissProt: P16106 Human
- SwissProt: Q16695 Human
- SwissProt: Q93081 Human
see all
Anti-Histone H3 (citrulline R26) antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
ELISA - Histone H3 (citrulline R26) antibody (ab19847)
ab19847 gave a positive result in ELISA against the immunising peptide (ab20631) and a negative result using the non-modified equivalent peptide (ab2623). This indicates that ab19847 is specifically recognising Histone H3 citrulline R26.
Green and Orange lines: ELISA against immunising peptide (ab20631)
Red and Mauve lines: ELISA against non-modified peptide (ab2623)
References for Anti-Histone H3 (citrulline R26) antibody (ab19847)
This product has been referenced in:
- Cherrington BDet al. Potential role for peptidylarginine deiminase 2 (PAD2) in citrullination of canine mammary epithelial cell histones. PLoS One 5:e11768 (2010). WB; Dog.Read more (PubMed: 20668670) »
See 1 publication for this product
Publishing research using ab19847? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"