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Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab9048 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

11 questions for ab9048

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Question 1

Thursday 23-February-2012

Dear Sir/Madam,

Are your antibodies for H3.3, H3K36me1, H3K36me2, H3K36me3 better diluted in milk or BSA for western blot?

Best wishes,

ANSWER:

  

Thank you for contacting us and your interest in our products.


Our anti-histone antibodiesare generally blocked with 5% BSA in 0.1% TBST for 1 hour at room temperature with gentle rocking when performing western blotting. We also use 5% BSA in the antibody diluentand use with overnight incubation at 4ºC. This procedure may need some optimisation with the particular sample and protocol used.


I hope this information has been of help, if you require any further information please do not hesitate to contact us again.

Question 2

Tuesday 21-February-2012

Thank you for the information. Could you please provide us with documentation for both of currently available lots. We would like to compare and choose the one which is the most specific to mono methyl K36 and with the highest affinity. ChIP data would be also very helpful.

ANSWER:

  

Thanks for your reply, and I apologize again for the delay getting this data to you.

Please see attached for the data for both lots, and if you have any questions or concerns please contact me and I'll be happy to help.

Have a great week!

Question 3

Monday 20-February-2012


Thank you for the information. Could you please provide us with documentation for both of currently available lots. We would like to compare and choose the one which is the most specific to mono methyl K36 and with the highest affinity. ChIP data would be also very helpful.
Thank you for your help,

ANSWER:

  

Sure, I will get in touch with the lab and request that information for you. I apologize for the delay, but I expect to have all of the data by tomorrow.

Please let me know if you need anything else in the meantime and I'll be happy to help.

Question 4

Monday 20-February-2012

Last week I asked for more information about specificity of these lots. Do you know if it is available?

Thank you,

ANSWER:

  

Thank you for your enquiry, and I apologize that it has taken so long to get this information for you.

Each new lot of ab9048 is tested in Western blot with blocking peptides, similarly to the blot shown on the datasheet, and is also tested in IF and ChIP.
I have attached the WB blocking results to this email.




I hope this information will be useful, but please let me know if there is anything else that you need and I'll be happy to help.

Question 5

Friday 10-March-2006

The experimental sample is another whole cell lysate of a different histone point mutation that i was hypothesizing was hypermethylated at K36. Presumably it should contain the same non-specific bands as the other samples so I am a little confused as to its identity. I am probing the blot now with an N-terminal histone H3 antibody to determine whether it is H3, however, if this is true there is not a good explanation as to why it is present when i probe with the mono methyl specific antibody. nonetheless i want to thank you for your input and i will gladly fill out the review sheet. :)

ANSWER:

  

Thank you for getting back in touch with me.

That is very strange that an alternative point mutation would have a total absence of lower band. It is indeed strange and must be lysate specific.

Thank you for leaving a review, I found your case most interesting.

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