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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H3, mono methylated at K4.
(Peptide available as ab1340.)
Our Abpromise guarantee covers the use of ab8895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Electron Microscopy||Use at an assay dependent dilution. PubMed: 20543957|
|ICC/IF||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent concentration.|
|ChIP||Use 2 µg for 25 µg of chromatin.|
|WB||1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human Histone H3 (mono methyl K4) peptide (ab1340).|
|CHIPseq||Use at an assay dependent concentration. PubMed: 22196736|
|IHC-P||Use at an assay dependent dilution.|
|ChIP/Chip||Use at an assay dependent dilution.|
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab8895 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and ALDOA (active) and MYO-D (inactive) promoters and over the y-Actin gene (active). Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.
Indian muntjac fibroblast cells - interphase (top left) and prophase (top right and below), stained with:
Mono Methyl K4 antibody, ab8895, (green)
DAPI: red, top left and right; blue, below
Phospho Ser 10 antibody: red (below) and blue (top right)
The perinuclear and perinucleolar heterochromatin domains do not contain Mono Methyl K4. The Mono Methyl K4, rather, is distributed as small nuclear foci primarily found between DAPI-intense regions of the nucleus.
ab8895 staining mouse embryonic stem cells by flow cytometry. The ES cell colonies were trypsinized and the cells permeabilized before being stained with ab8895 (0.25ug/1.5 x 105 cells). A PE conjugated goat anti-rabbit antibody was used as the secondary.
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