Histone H3 peptide - di methyl K9 (ab1772)
Our Abpromise guarantee covers the use of ab1772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Coupling to beads via C-term Cys residues.
Affinity purifying factors from cell extracts inorder to identify specific binding proteins.
Substrates in methytransferase oracetyltransferase assays (or ribosyltransferase/kinase assays).
Competitors to challange interactions observed inco-IPs or by immunofluorescence.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FLJ92264H3 histoneH3 histone
- H3 histone family, member AH3/aH3/bH3/cH3/dh3/fH3/hH3/iH3/jH3/kH3/lH31_HUMANH3F1KH3F3H3FAH3FBH3FCH3FDH3FFH3FHH3FIH3FJH3FKH3FLHIST1H3AHIST1H3BHIST1H3CHIST1H3DHIST1H3EHIST1H3FHIST1H3GHIST1H3HHIST1H3IHIST1H3JHIST3H3Histone 1, H3aHistone cluster 1, H3aHistone cluster 1, H3bHistone cluster 1, H3cHistone cluster 1, H3dHistone cluster 1, H3eHistone cluster 1, H3fHistone cluster 1, H3gHistone cluster 1, H3iHistone cluster 1, H3jHistone H 3Histone H3.1Histone H3.1Histone H3/aHistone H3/bHistone H3/cHistone H3/dHistone H3/fHistone H3/hHistone H3/iHistone H3/jHistone H3/kHistone H3/l
modificationsAcetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
Histone H3 peptide - di methyl K9 images
All lanes : Anti-Histone H3 (methylated) antibody [mAbcam 1220] - ChIP Grade (ab1220)
Lane 1 : Calf thymus histone lysate
Lane 2 : Calf thymus histone lysate with Histone H3 peptide - unmodified at 1 µg/ml
Lane 3 : Calf thymus histone lysate with
Histone H3 peptide - mono methyl K9 (ab1771) at 1 µg/ml
Lane 4 : Calf thymus histone lysate with
Histone H3 peptide - di methyl K9 (ab1772) at 1 µg/ml
Lane 5 : Calf thymus histone lysate with
Histone H3 peptide - tri methyl K9 (ab1773) at 1 µg/ml
Lane 6 : Calf thymus histone lysate with
Histone H3 peptide - di methyl K4 (ab7768) at 1 µg/ml
Lane 7 : Calf thymus histone lysate with
Histone H3 peptide - di methyl K27 (ab1781) at 1 µg/ml
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 17 kDa
Exposure time : 1 minute
References for Histone H3 peptide - di methyl K9 (ab1772)
This product has been referenced in:
- Beyer S et al. The histone demethylases JMJD1A and JMJD2B are transcriptional targets of hypoxia-inducible factor HIF. J Biol Chem 283:36542-52 (2008). Read more (PubMed: 18984585) »
- Joshi AA & Struhl K Eaf3 chromodomain interaction with methylated H3-K36 links histone deacetylation to Pol II elongation. Mol Cell 20:971-8 (2005). Read more (PubMed: 16364921) »