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Anti-Histone H3 (phospho T3, tri methyl K4) antibody (ab3738)

This fast track antibody is not yet fully characterised. It is subject to these terms and conditions

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We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.

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Overview

Product name

Anti-Histone H3 (phospho T3, tri methyl K4) antibody
See all Histone H3 products (2) ...

Description

Rabbit polyclonal to Histone H3 (phospho T3, tri methyl K4)

This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

Cross reactivity

Predicted to work with

Human, a wide range of other species

Immunogen

Synthetic peptide derived from within residues 1 - 100 of Human Histone H3, phosphorylated at threonine 3 and tri-methylated at lysine 4.

Read Abcam's proprietary immunogen policy

(Peptides available as ab206 35 and ab1342 .)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Histones >> H3 >> Methyl + Phospho

Applications

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Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).Fast track terms of use

  • WB

     

Application notes

ELISA: Use at an assay dependent dilution. By ELISA this antibody looks specific to the dual modification with no cross reactivity with either of the single modifications in ELISA.

This antibody gave a positive result in ELISA against the immunising peptide.

For the following peptides it gave the results shown: the non-modified equivalent peptide (ab14664) - negative result, Histone H3 tri methyl K9 peptide (ab1342) - negative result, Histone H3 phospho T3 (ab17578) -negative result.

This indicates that it is specific for the dual modified peptide.


Is unsuitable for WB. Unfortunately we are unable to get an image in WB and this could be due to this dual modified histone not being expressed in our normal histone lysate.


Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Cellular localization

Nuclear

Database links

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References for Anti-Histone H3 (phospho T3, tri methyl K4) antibody (ab3738)

ab3738 has not yet been referenced specifically in any publications.

Publishing research using ab3738? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"