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Products:Epigenetics and Nuclear Signaling >> Histones >> H3 >> Methylated
Anti-Histone H3 (tri methyl K4) antibody (ab71998)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab71998 for help.
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We purchased the antibody (AB71998)and planned to label this antibody with one fluorescence dye. So it is critical to us to make clear what the components are in the storage buffer. In your website, it says "Constituents: PBS, pH 7.2 containing antibody stabilizer". What is actually the antibody stabilizer? Please let me know. |
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ANSWER: |
The antibody stabilizer has BSA, Gycerol, Procline300. It can’t be used for dye conjugation. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - Histone H3 (tri methyl K4) antibody (ab71998)
ab71998 at 1/100 dilution staining Histone H3 in 3T3 cells by Immunocytochemistry/ Immunofluorescence. A FITC labeled goat anti Rb IgG was used for visualization under fluorescent microscope.
Western blot - Histone H3 (tri methyl K4) antibody (ab71998)
All lanes : Anti-Histone H3 (tri methyl K4) antibody (ab71998) at 1/1000 dilution
Lane 1 : 3T3 nuclear extract immunoprecipitated
using 4 µg ab71998
Lane 2 : 3T3 nuclear extract immunoprecipitated
using control Rabbit IgG
Predicted band size : 15 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Additional bands at : 22 kDa (possible IgG),50 kDa (possible IgG).
For IP 5 mg/ml of cell nuclear extract was incubated with 4 ug of antibody 4oC O/N and pulled down by Protein A beads. Samples were then boiled in SDS-PAGE sample buffer and loaded into 12% SDS-PAGE. Transfer was onto NC membrane and blotted with the same antibody at 1:1000.
ChIP - Histone H3 (tri methyl K4) antibody (ab71998)
Chromatin was prepared from Jurkat cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab71998 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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